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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1281 | 5' | -57.5 | NC_001317.1 | + | 10980 | 0.66 | 0.415773 |
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Target: 5'- aAUCUCgGGGCGAauuuCAAuguuGAGCGCCuGGu -3' miRNA: 3'- -UGGAG-CCCGCU----GUUu---CUCGCGGuCUg -5' |
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| 1281 | 5' | -57.5 | NC_001317.1 | + | 17866 | 0.68 | 0.317622 |
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Target: 5'- aACCUCGgcGGCGACuuuAAAGAGUuCCAGuCu -3' miRNA: 3'- -UGGAGC--CCGCUG---UUUCUCGcGGUCuG- -5' |
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| 1281 | 5' | -57.5 | NC_001317.1 | + | 12249 | 0.69 | 0.2784 |
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Target: 5'- gGCCguuGGUGACGGAGgcggaaaacugccAGUGCCGGACg -3' miRNA: 3'- -UGGagcCCGCUGUUUC-------------UCGCGGUCUG- -5' |
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| 1281 | 5' | -57.5 | NC_001317.1 | + | 16511 | 0.69 | 0.274769 |
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Target: 5'- aGCCugUCGGGCGAUAuucgcgacacacaaaAAGGGCuGCguGACc -3' miRNA: 3'- -UGG--AGCCCGCUGU---------------UUCUCG-CGguCUG- -5' |
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| 1281 | 5' | -57.5 | NC_001317.1 | + | 5063 | 1.08 | 0.000297 |
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Target: 5'- cACCUCGGGCGACAAAGAGCGCCAGACc -3' miRNA: 3'- -UGGAGCCCGCUGUUUCUCGCGGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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