Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12816 | 5' | -58.8 | NC_003387.1 | + | 13618 | 0.66 | 0.479114 |
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Target: 5'- gCGCUGGCaCCGGGuGAGUUcaACCcGGc -3' miRNA: 3'- -GUGGCCGcGGCCCuCUCAA--UGGaCUa -5' |
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| 12816 | 5' | -58.8 | NC_003387.1 | + | 30506 | 0.67 | 0.449476 |
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Target: 5'- uGCCGGgGCCGGGgccgcgAGAGcccGCUUGGg -3' miRNA: 3'- gUGGCCgCGGCCC------UCUCaa-UGGACUa -5' |
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| 12816 | 5' | -58.8 | NC_003387.1 | + | 72 | 0.68 | 0.358636 |
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Target: 5'- gAUCGGCGCCGcGGGcGUUugCUGGc -3' miRNA: 3'- gUGGCCGCGGCcCUCuCAAugGACUa -5' |
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| 12816 | 5' | -58.8 | NC_003387.1 | + | 45972 | 0.72 | 0.223779 |
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Target: 5'- uCGCCGGUGCC--GAGGGUgGCCUGGUc -3' miRNA: 3'- -GUGGCCGCGGccCUCUCAaUGGACUA- -5' |
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| 12816 | 5' | -58.8 | NC_003387.1 | + | 39237 | 0.73 | 0.17647 |
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Target: 5'- gUACCGGCGCCGGGcGAG--GCCgucGAg -3' miRNA: 3'- -GUGGCCGCGGCCCuCUCaaUGGa--CUa -5' |
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| 12816 | 5' | -58.8 | NC_003387.1 | + | 30391 | 1.05 | 0.000819 |
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Target: 5'- cCACCGGCGCCGGGAGAGUUACCUGAUc -3' miRNA: 3'- -GUGGCCGCGGCCCUCUCAAUGGACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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