Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12818 | 5' | -51.9 | NC_003387.1 | + | 4336 | 0.68 | 0.729765 |
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Target: 5'- cUGCGcCCGGCCcgCUUGCGAAuGUgGUUg -3' miRNA: 3'- -AUGC-GGCUGGa-GAACGCUU-UAgCAGu -5' |
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| 12818 | 5' | -51.9 | NC_003387.1 | + | 7940 | 0.69 | 0.718791 |
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Target: 5'- cGCGCgGGCCagCUUGuCGAGGUCGg-- -3' miRNA: 3'- aUGCGgCUGGa-GAAC-GCUUUAGCagu -5' |
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| 12818 | 5' | -51.9 | NC_003387.1 | + | 43244 | 0.69 | 0.674101 |
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Target: 5'- gACGCCGuaCUCgggGuCGAAAUCGUCGg -3' miRNA: 3'- aUGCGGCugGAGaa-C-GCUUUAGCAGU- -5' |
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| 12818 | 5' | -51.9 | NC_003387.1 | + | 28071 | 0.7 | 0.640096 |
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Target: 5'- gGCGCuggCGACCUCgcgcagcaggGCGAGGUgCGUCGg -3' miRNA: 3'- aUGCG---GCUGGAGaa--------CGCUUUA-GCAGU- -5' |
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| 12818 | 5' | -51.9 | NC_003387.1 | + | 35897 | 0.71 | 0.572156 |
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Target: 5'- cACGUCGACCUgcUGCGGua-CGUCAa -3' miRNA: 3'- aUGCGGCUGGAgaACGCUuuaGCAGU- -5' |
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| 12818 | 5' | -51.9 | NC_003387.1 | + | 4812 | 0.71 | 0.565432 |
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Target: 5'- -cCGCCGACCagcgcgugcgcaagcUCgUGCGuAAUCGUCAu -3' miRNA: 3'- auGCGGCUGG---------------AGaACGCuUUAGCAGU- -5' |
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| 12818 | 5' | -51.9 | NC_003387.1 | + | 50465 | 0.74 | 0.404206 |
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Target: 5'- --aGCCGACCcCggcccGCGAGGUCGUCGg -3' miRNA: 3'- augCGGCUGGaGaa---CGCUUUAGCAGU- -5' |
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| 12818 | 5' | -51.9 | NC_003387.1 | + | 21719 | 0.76 | 0.316286 |
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Target: 5'- cGCGCCGACCUggucgUG-GAAAUCGUCGc -3' miRNA: 3'- aUGCGGCUGGAga---ACgCUUUAGCAGU- -5' |
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| 12818 | 5' | -51.9 | NC_003387.1 | + | 29275 | 1.07 | 0.002625 |
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Target: 5'- uUACGCCGACCUCUUGCGAAAUCGUCAu -3' miRNA: 3'- -AUGCGGCUGGAGAACGCUUUAGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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