Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12819 | 3' | -58.4 | NC_003387.1 | + | 26855 | 0.66 | 0.512368 |
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Target: 5'- -cCAGgc-CGCUCGGCaacugcaggccaCCCUUGCCGc -3' miRNA: 3'- cuGUCaaaGCGAGCCG------------GGGAGCGGUu -5' |
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| 12819 | 3' | -58.4 | NC_003387.1 | + | 23128 | 0.66 | 0.491631 |
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Target: 5'- cGACAuagUCGCccggcucCGGCCCCUCgGCCu- -3' miRNA: 3'- -CUGUcaaAGCGa------GCCGGGGAG-CGGuu -5' |
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| 12819 | 3' | -58.4 | NC_003387.1 | + | 51305 | 0.66 | 0.481409 |
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Target: 5'- cGCAGgaUCGCUCGGgCCgaCGuCCGGu -3' miRNA: 3'- cUGUCaaAGCGAGCCgGGgaGC-GGUU- -5' |
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| 12819 | 3' | -58.4 | NC_003387.1 | + | 23233 | 0.66 | 0.471291 |
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Target: 5'- cGGCuGUggUGCUCGGCgCCCUCGg--- -3' miRNA: 3'- -CUGuCAaaGCGAGCCG-GGGAGCgguu -5' |
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| 12819 | 3' | -58.4 | NC_003387.1 | + | 45937 | 0.68 | 0.37584 |
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Target: 5'- cGACcauGUcgCGCUCGGCCagguugaucugguUCUCGCCGGu -3' miRNA: 3'- -CUGu--CAaaGCGAGCCGG-------------GGAGCGGUU- -5' |
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| 12819 | 3' | -58.4 | NC_003387.1 | + | 185 | 0.68 | 0.359396 |
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Target: 5'- aGACGGUgcUCGaCUCGGCCCgcgacaUUGCCGc -3' miRNA: 3'- -CUGUCAa-AGC-GAGCCGGGg-----AGCGGUu -5' |
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| 12819 | 3' | -58.4 | NC_003387.1 | + | 3493 | 0.72 | 0.200726 |
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Target: 5'- aGCAGc--CGCUCGGCCgCCUCGaCCGg -3' miRNA: 3'- cUGUCaaaGCGAGCCGG-GGAGC-GGUu -5' |
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| 12819 | 3' | -58.4 | NC_003387.1 | + | 19748 | 0.73 | 0.190274 |
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Target: 5'- gGGCAcGUagUCGCgCGGCUCCUCGCCu- -3' miRNA: 3'- -CUGU-CAa-AGCGaGCCGGGGAGCGGuu -5' |
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| 12819 | 3' | -58.4 | NC_003387.1 | + | 28181 | 1.06 | 0.000623 |
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Target: 5'- cGACAGUUUCGCUCGGCCCCUCGCCAAg -3' miRNA: 3'- -CUGUCAAAGCGAGCCGGGGAGCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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