Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1282 | 3' | -54.3 | NC_001317.1 | + | 12699 | 0.66 | 0.633796 |
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Target: 5'- aGAcAGCGCgucUGAGgUGCUUGCGGCGa -3' miRNA: 3'- cCUuUUGUG---GCUCaGCGAGCGCCGCc -5' |
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| 1282 | 3' | -54.3 | NC_001317.1 | + | 798 | 0.67 | 0.554906 |
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Target: 5'- aGAAA--ACCGAaucGUCGacccCGCGGCGGg -3' miRNA: 3'- cCUUUugUGGCU---CAGCga--GCGCCGCC- -5' |
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| 1282 | 3' | -54.3 | NC_001317.1 | + | 10448 | 0.68 | 0.543826 |
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Target: 5'- ---uGACGCCG-GUCgGCUCGCgGGUGa -3' miRNA: 3'- ccuuUUGUGGCuCAG-CGAGCG-CCGCc -5' |
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| 1282 | 3' | -54.3 | NC_001317.1 | + | 14828 | 0.68 | 0.543826 |
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Target: 5'- ------gACCGAGUgCGCggGCGGUGGc -3' miRNA: 3'- ccuuuugUGGCUCA-GCGagCGCCGCC- -5' |
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| 1282 | 3' | -54.3 | NC_001317.1 | + | 13238 | 0.68 | 0.511056 |
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Target: 5'- cGGAAAUAUCGAGggggCGCgauGgGGCGGu -3' miRNA: 3'- cCUUUUGUGGCUCa---GCGag-CgCCGCC- -5' |
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| 1282 | 3' | -54.3 | NC_001317.1 | + | 9870 | 0.69 | 0.468712 |
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Target: 5'- ----cGCGCCG-GUCGUUCGCgcacauGGUGGg -3' miRNA: 3'- ccuuuUGUGGCuCAGCGAGCG------CCGCC- -5' |
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| 1282 | 3' | -54.3 | NC_001317.1 | + | 2496 | 0.69 | 0.468712 |
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Target: 5'- cGAcAGCGCCGGGUgcgucgUGCUCGCgccgccGGUGGu -3' miRNA: 3'- cCUuUUGUGGCUCA------GCGAGCG------CCGCC- -5' |
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| 1282 | 3' | -54.3 | NC_001317.1 | + | 11389 | 0.72 | 0.320284 |
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Target: 5'- cGGAAAAUgaGCCGAuUCGU--GCGGCGGc -3' miRNA: 3'- -CCUUUUG--UGGCUcAGCGagCGCCGCC- -5' |
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| 1282 | 3' | -54.3 | NC_001317.1 | + | 5578 | 1.13 | 0.000362 |
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Target: 5'- aGGAAAACACCGAGUCGCUCGCGGCGGa -3' miRNA: 3'- -CCUUUUGUGGCUCAGCGAGCGCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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