miRNA: 3'- -UGCUUUGCG--------UCGUGG---GCCAa---GAGCUG- -5'
miRNA: 3'- -UGCUU----UGCGuC-GUGG-GCCaa--GAGCUG- -5'
miRNA: 3'- -UGCUUugCGUCG-----UGGGCCaagaGCUg -5'
miRNA: 3'- -UGCUUu---GCGUCGUGGGccaAGaGCUG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12823 | 3' | -54.3 | NC_003387.1 | + | 50241 | 0.71 | 0.46973 |
|
Target: 5'- uACGAGGCGCcguacgucAGCGCCgggCGGUgcacCUCGGCc -3' miRNA: 3'- -UGCUUUGCG--------UCGUGG---GCCAa---GAGCUG- -5' |
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| 12823 | 3' | -54.3 | NC_003387.1 | + | 50663 | 0.66 | 0.77214 |
|
Target: 5'- cACGAAcaugGCGCcGcCACCgCGGccagCUCGGCg -3' miRNA: 3'- -UGCUU----UGCGuC-GUGG-GCCaa--GAGCUG- -5' |
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| 12823 | 3' | -54.3 | NC_003387.1 | + | 52574 | 0.66 | 0.751483 |
|
Target: 5'- cGCGAGugGUAGCaaaccGCCCGGcgagCGAg -3' miRNA: 3'- -UGCUUugCGUCG-----UGGGCCaagaGCUg -5' |
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| 12823 | 3' | -54.3 | NC_003387.1 | + | 52716 | 0.66 | 0.751483 |
|
Target: 5'- cGCGAAcgauCGCGGCGCCCc--UCgCGGCa -3' miRNA: 3'- -UGCUUu---GCGUCGUGGGccaAGaGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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