miRNA: 3'- uGCUuuGCGUcGUGGGCCaaGAgCUG- -5'
miRNA: 3'- uGCuuUGCGU--CG-------UGGGc---CAAGAGCUG- -5'
miRNA: 3'- -UGCUU--UgCGUCGU---GGGCCAag---AGCUG- -5'
miRNA: 3'- -UGCU--UUGCG---UCGUGgGCCAa--GAGCUG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12823 | 3' | -54.3 | NC_003387.1 | + | 2466 | 0.67 | 0.697879 |
|
Target: 5'- cCGAggUGCAcCGCCCGGcgCU-GACg -3' miRNA: 3'- uGCUuuGCGUcGUGGGCCaaGAgCUG- -5' |
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| 12823 | 3' | -54.3 | NC_003387.1 | + | 2413 | 0.69 | 0.575019 |
|
Target: 5'- uCGggGCGCAucGCguugacgGCCCagccGUUCUCGACg -3' miRNA: 3'- uGCuuUGCGU--CG-------UGGGc---CAAGAGCUG- -5' |
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| 12823 | 3' | -54.3 | NC_003387.1 | + | 1720 | 0.68 | 0.631518 |
|
Target: 5'- aGCGGGccGgGCGGCGcauCCCGGUgcugaUCGACa -3' miRNA: 3'- -UGCUU--UgCGUCGU---GGGCCAag---AGCUG- -5' |
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| 12823 | 3' | -54.3 | NC_003387.1 | + | 1116 | 0.69 | 0.598199 |
|
Target: 5'- cAUGAgcGACGCuagGGCGCaCGGUgugCUCGGCg -3' miRNA: 3'- -UGCU--UUGCG---UCGUGgGCCAa--GAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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