Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12835 | 3' | -50.5 | NC_003387.1 | + | 39060 | 0.67 | 0.870405 |
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Target: 5'- uCGUGCuuacucacgguugACCACGUcGCGGuCGGgccgUUCg -3' miRNA: 3'- -GCACG-------------UGGUGCA-UGCC-GCCaauaAAG- -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 42230 | 0.66 | 0.921588 |
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Target: 5'- --cGCACCGCG-AUGGgcgaGGUUGUgUCg -3' miRNA: 3'- gcaCGUGGUGCaUGCCg---CCAAUAaAG- -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 42746 | 0.67 | 0.871223 |
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Target: 5'- gGUGCGCC-C-UGCGGCGGa------ -3' miRNA: 3'- gCACGUGGuGcAUGCCGCCaauaaag -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 44461 | 0.71 | 0.668254 |
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Target: 5'- uGUGCACCu--UGCGGUGGUUGg--- -3' miRNA: 3'- gCACGUGGugcAUGCCGCCAAUaaag -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 46470 | 0.66 | 0.927621 |
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Target: 5'- uCGUGCACCGgGaugaugACGGCG---AUUUCc -3' miRNA: 3'- -GCACGUGGUgCa-----UGCCGCcaaUAAAG- -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 48437 | 0.66 | 0.933358 |
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Target: 5'- --aGCACCGCGUGCaGUucaucgagGGUUAcgUCg -3' miRNA: 3'- gcaCGUGGUGCAUGcCG--------CCAAUaaAG- -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 48728 | 0.66 | 0.932798 |
|
Target: 5'- cCGUGCGCCGCG-ACGaguccaugaccgcGCGGg----UCg -3' miRNA: 3'- -GCACGUGGUGCaUGC-------------CGCCaauaaAG- -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 51353 | 0.67 | 0.90172 |
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Target: 5'- -uUGCGCCAC-UGCGaGCGGUcg--UCg -3' miRNA: 3'- gcACGUGGUGcAUGC-CGCCAauaaAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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