Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12835 | 3' | -50.5 | NC_003387.1 | + | 2368 | 0.69 | 0.787996 |
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Target: 5'- --aGCGCCACGUAC-GCGGUc----- -3' miRNA: 3'- gcaCGUGGUGCAUGcCGCCAauaaag -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 34208 | 0.69 | 0.777728 |
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Target: 5'- --aGCGCCACGUcGCGGuCGGUg----- -3' miRNA: 3'- gcaCGUGGUGCA-UGCC-GCCAauaaag -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 31024 | 0.7 | 0.72422 |
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Target: 5'- gCGUGCGCCGgG-GCGGCGGc------ -3' miRNA: 3'- -GCACGUGGUgCaUGCCGCCaauaaag -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 20347 | 0.71 | 0.713173 |
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Target: 5'- uGUGUcggccagcccccGCCuCGUGCGGgGGUUGUgUCg -3' miRNA: 3'- gCACG------------UGGuGCAUGCCgCCAAUAaAG- -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 23966 | 0.71 | 0.70204 |
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Target: 5'- aCGUGCGCaGCGccggGCGGCuGGUUGUg-- -3' miRNA: 3'- -GCACGUGgUGCa---UGCCG-CCAAUAaag -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 44461 | 0.71 | 0.668254 |
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Target: 5'- uGUGCACCu--UGCGGUGGUUGg--- -3' miRNA: 3'- gCACGUGGugcAUGCCGCCAAUaaag -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 37741 | 0.75 | 0.478893 |
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Target: 5'- uGUGCGCCAcCGUgcGCGGCGGg------ -3' miRNA: 3'- gCACGUGGU-GCA--UGCCGCCaauaaag -5' |
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| 12835 | 3' | -50.5 | NC_003387.1 | + | 21907 | 1.11 | 0.002184 |
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Target: 5'- uCGUGCACCACGUACGGCGGUUAUUUCg -3' miRNA: 3'- -GCACGUGGUGCAUGCCGCCAAUAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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