Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12846 | 5' | -57.9 | NC_003387.1 | + | 23833 | 0.66 | 0.498151 |
Target: 5'- -gGgCGCCGCGcCGGGAcucgGcGCCGUGc -3' miRNA: 3'- caCgGCGGCGC-GCCCUa---CuUGGUAUa -5' |
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12846 | 5' | -57.9 | NC_003387.1 | + | 40453 | 0.66 | 0.508547 |
Target: 5'- -gGCCGCCggucgccagGCGCGGGAUauucgGGAUCGg-- -3' miRNA: 3'- caCGGCGG---------CGCGCCCUA-----CUUGGUaua -5' |
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12846 | 5' | -57.9 | NC_003387.1 | + | 30583 | 0.66 | 0.519034 |
Target: 5'- cGUGCCGCgaGUGCGGGuacgucGGCCAc-- -3' miRNA: 3'- -CACGGCGg-CGCGCCCuac---UUGGUaua -5' |
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12846 | 5' | -57.9 | NC_003387.1 | + | 17089 | 0.66 | 0.540254 |
Target: 5'- aGUGCCcgcccgGCCGCcucgGCGGGcuccucgGGGCCGUAg -3' miRNA: 3'- -CACGG------CGGCG----CGCCCua-----CUUGGUAUa -5' |
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12846 | 5' | -57.9 | NC_003387.1 | + | 14233 | 0.66 | 0.540254 |
Target: 5'- -cGCCcacgGCCGCuCGGGAUcGGGCCGa-- -3' miRNA: 3'- caCGG----CGGCGcGCCCUA-CUUGGUaua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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