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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1285 | 3' | -51.8 | NC_001317.1 | + | 18165 | 0.67 | 0.708233 |
Target: 5'- aCCGGUagUGCcauuGUGACCGCaCUUggCGCGa -3' miRNA: 3'- -GGCUA--AUGu---CGCUGGUG-GGAaaGCGC- -5' |
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1285 | 3' | -51.8 | NC_001317.1 | + | 19178 | 0.67 | 0.687681 |
Target: 5'- gCCGAguuuuucagcgACGGCGcuGCCagaaagauugauuucACCCUUUCGCu -3' miRNA: 3'- -GGCUaa---------UGUCGC--UGG---------------UGGGAAAGCGc -5' |
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1285 | 3' | -51.8 | NC_001317.1 | + | 7872 | 1.11 | 0.000919 |
Target: 5'- aCCGAUUACAGCGACCACCCUUUCGCGc -3' miRNA: 3'- -GGCUAAUGUCGCUGGUGGGAAAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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