Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1286 | 3' | -51.1 | NC_001317.1 | + | 16221 | 0.66 | 0.748123 |
Target: 5'- gUGGUGAGUgCCGgGUAGGUcAUGCgggGCa -3' miRNA: 3'- -AUUACUUA-GGCgCGUCCAcUGUGa--CG- -5' |
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1286 | 3' | -51.1 | NC_001317.1 | + | 1819 | 0.67 | 0.701997 |
Target: 5'- ----cAAUCUgGCGCGauuuGGUGAUGCUGCg -3' miRNA: 3'- auuacUUAGG-CGCGU----CCACUGUGACG- -5' |
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1286 | 3' | -51.1 | NC_001317.1 | + | 2412 | 0.67 | 0.678393 |
Target: 5'- -cAUGGAcuuugCCGCGCAGGcagaUGGCAUccGCa -3' miRNA: 3'- auUACUUa----GGCGCGUCC----ACUGUGa-CG- -5' |
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1286 | 3' | -51.1 | NC_001317.1 | + | 8244 | 0.67 | 0.666506 |
Target: 5'- -uGUGAuggCCGUGCucGGGUugcuGugGCUGCa -3' miRNA: 3'- auUACUua-GGCGCG--UCCA----CugUGACG- -5' |
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1286 | 3' | -51.1 | NC_001317.1 | + | 6112 | 0.69 | 0.571131 |
Target: 5'- cUGAUG-GUCguggUGCGCAGGaUGACAUUGUu -3' miRNA: 3'- -AUUACuUAG----GCGCGUCC-ACUGUGACG- -5' |
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1286 | 3' | -51.1 | NC_001317.1 | + | 15032 | 0.69 | 0.558182 |
Target: 5'- ---cGAA-CCGCGCAGGUgcuggccGACACaaugGCu -3' miRNA: 3'- auuaCUUaGGCGCGUCCA-------CUGUGa---CG- -5' |
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1286 | 3' | -51.1 | NC_001317.1 | + | 28259 | 0.69 | 0.547649 |
Target: 5'- --uUGAAUUCGcCGCAGuGcaUGAUGCUGCu -3' miRNA: 3'- auuACUUAGGC-GCGUC-C--ACUGUGACG- -5' |
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1286 | 3' | -51.1 | NC_001317.1 | + | 9783 | 0.71 | 0.479356 |
Target: 5'- gUGGUGAcggaggucgAUaCCGUGCAGG-GGCugUGCc -3' miRNA: 3'- -AUUACU---------UA-GGCGCGUCCaCUGugACG- -5' |
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1286 | 3' | -51.1 | NC_001317.1 | + | 3307 | 0.71 | 0.457513 |
Target: 5'- cAGUGGuaCCGCGCAGGacUGGCACa-- -3' miRNA: 3'- aUUACUuaGGCGCGUCC--ACUGUGacg -5' |
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1286 | 3' | -51.1 | NC_001317.1 | + | 3920 | 0.73 | 0.330186 |
Target: 5'- ---cGAAUCgGCGUcgaGGGUGAUAcCUGCg -3' miRNA: 3'- auuaCUUAGgCGCG---UCCACUGU-GACG- -5' |
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1286 | 3' | -51.1 | NC_001317.1 | + | 16143 | 0.73 | 0.330186 |
Target: 5'- --cUGAcgCCGCGCAGGguGC-CUGCg -3' miRNA: 3'- auuACUuaGGCGCGUCCacUGuGACG- -5' |
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1286 | 3' | -51.1 | NC_001317.1 | + | 2069 | 0.74 | 0.304767 |
Target: 5'- cGGUGcggcuggCCuCGUAGGUGGCGCUGCg -3' miRNA: 3'- aUUACuua----GGcGCGUCCACUGUGACG- -5' |
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1286 | 3' | -51.1 | NC_001317.1 | + | 8396 | 1.12 | 0.000706 |
Target: 5'- gUAAUGAAUCCGCGCAGGUGACACUGCg -3' miRNA: 3'- -AUUACUUAGGCGCGUCCACUGUGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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