Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1287 | 5' | -57.2 | NC_001317.1 | + | 2896 | 0.66 | 0.443576 |
Target: 5'- --cGCAGaugcuucUGCGAGGCcaUGCCCGagGCg -3' miRNA: 3'- acaCGUC-------AUGCUCCGc-GCGGGCaaCG- -5' |
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1287 | 5' | -57.2 | NC_001317.1 | + | 10316 | 0.66 | 0.405294 |
Target: 5'- gGUGguGUGCaaaGAGGCGgUGCCUGg--- -3' miRNA: 3'- aCACguCAUG---CUCCGC-GCGGGCaacg -5' |
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1287 | 5' | -57.2 | NC_001317.1 | + | 2070 | 0.67 | 0.386487 |
Target: 5'- gGUGCGgcuggccucGUAgGuGGCgcuGCGCCCGcUGCu -3' miRNA: 3'- aCACGU---------CAUgCuCCG---CGCGGGCaACG- -5' |
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1287 | 5' | -57.2 | NC_001317.1 | + | 9616 | 0.67 | 0.359376 |
Target: 5'- --aGCA--ACG-GGCGCGCCuCGUacUGCa -3' miRNA: 3'- acaCGUcaUGCuCCGCGCGG-GCA--ACG- -5' |
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1287 | 5' | -57.2 | NC_001317.1 | + | 24767 | 0.68 | 0.317219 |
Target: 5'- -aUGCAGaaaccucaugGCGAcGGCuGCGCgCGUUGCu -3' miRNA: 3'- acACGUCa---------UGCU-CCG-CGCGgGCAACG- -5' |
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1287 | 5' | -57.2 | NC_001317.1 | + | 3577 | 0.69 | 0.26463 |
Target: 5'- --aGCAGccuUGAGGCGCGCCUGa--- -3' miRNA: 3'- acaCGUCau-GCUCCGCGCGGGCaacg -5' |
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1287 | 5' | -57.2 | NC_001317.1 | + | 9397 | 0.7 | 0.257722 |
Target: 5'- cGUGCGGcaUACGGGGUGCcgucggguGCCUGcugGCg -3' miRNA: 3'- aCACGUC--AUGCUCCGCG--------CGGGCaa-CG- -5' |
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1287 | 5' | -57.2 | NC_001317.1 | + | 17122 | 0.7 | 0.244349 |
Target: 5'- -aUGCAGgcguUACGcaaacAGGCGCGCCaCGUcgGCg -3' miRNA: 3'- acACGUC----AUGC-----UCCGCGCGG-GCAa-CG- -5' |
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1287 | 5' | -57.2 | NC_001317.1 | + | 17200 | 0.77 | 0.073698 |
Target: 5'- -cUGCAGUauugucgccgACGuGGCGCGCCUGuUUGCg -3' miRNA: 3'- acACGUCA----------UGCuCCGCGCGGGC-AACG- -5' |
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1287 | 5' | -57.2 | NC_001317.1 | + | 9559 | 1.11 | 0.000185 |
Target: 5'- aUGUGCAGUACGAGGCGCGCCCGUUGCu -3' miRNA: 3'- -ACACGUCAUGCUCCGCGCGGGCAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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