miRNA: 3'- -GGCaGCaCGACAACUauGgAGUUGUG- -5'
miRNA: 3'- -GGCAGCAcGACa---------ACUAUGGa--GUUGUG- -5'
miRNA: 3'- -GGCAgCACGACAACUAUGGAGUUGUg -5'
miRNA: 3'- -GGcAGCACGACAACUAUGGAGUUGUg -5'
miRNA: 3'- -GGCAGCACGACAACUAUGGAGUUGUG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12872 | 3' | -49.1 | NC_003387.1 | + | 22835 | 0.72 | 0.736829 |
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Target: 5'- aCCG-CGgGCUGUUGAccCgUCAACACg -3' miRNA: 3'- -GGCaGCaCGACAACUauGgAGUUGUG- -5' |
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| 12872 | 3' | -49.1 | NC_003387.1 | + | 42650 | 0.73 | 0.692182 |
|
Target: 5'- aCCGUCGUcCUGgucgccgccgUGAUACCggcCGACACc -3' miRNA: 3'- -GGCAGCAcGACa---------ACUAUGGa--GUUGUG- -5' |
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| 12872 | 3' | -49.1 | NC_003387.1 | + | 22805 | 0.96 | 0.032994 |
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Target: 5'- cCCGUaGUGCUGUUGAUGCCUCAACAg -3' miRNA: 3'- -GGCAgCACGACAACUAUGGAGUUGUg -5' |
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| 12872 | 3' | -49.1 | NC_003387.1 | + | 2123 | 0.96 | 0.031028 |
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Target: 5'- cCCcUCGUGCUGUUGAUACCUCAACAg -3' miRNA: 3'- -GGcAGCACGACAACUAUGGAGUUGUg -5' |
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| 12872 | 3' | -49.1 | NC_003387.1 | + | 2713 | 1.13 | 0.002511 |
|
Target: 5'- cCCGUCGUGCUGUUGAUACCUCAACACu -3' miRNA: 3'- -GGCAGCACGACAACUAUGGAGUUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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