Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1288 | 5' | -51 | NC_001317.1 | + | 14119 | 1.12 | 0.000804 |
Target: 5'- aAGACCAGCCAGGACAAUAACACUCGCc -3' miRNA: 3'- -UCUGGUCGGUCCUGUUAUUGUGAGCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 10820 | 0.76 | 0.26472 |
Target: 5'- aAGACCAG-CAGGAagcuguagcccgGACGCUCGCa -3' miRNA: 3'- -UCUGGUCgGUCCUguua--------UUGUGAGCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 22560 | 0.74 | 0.319058 |
Target: 5'- cGACUAGCCAuaagccgucaGACAAUGAUGCUUGCu -3' miRNA: 3'- uCUGGUCGGUc---------CUGUUAUUGUGAGCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 20203 | 0.71 | 0.485965 |
Target: 5'- uGGCCGGUgAGGcCGAUAACguGCUgGCg -3' miRNA: 3'- uCUGGUCGgUCCuGUUAUUG--UGAgCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 27513 | 0.68 | 0.636772 |
Target: 5'- -cGCCAGUCGGuGACGcaGUuuuUACUCGCg -3' miRNA: 3'- ucUGGUCGGUC-CUGU--UAuu-GUGAGCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 14661 | 0.68 | 0.648649 |
Target: 5'- aAGACCAuuuCCGaGGCGAUGGCAUaUCGCg -3' miRNA: 3'- -UCUGGUc--GGUcCUGUUAUUGUG-AGCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 2837 | 0.68 | 0.672337 |
Target: 5'- cAGACCAGaaGGGAUAA--GCGCcgUGCg -3' miRNA: 3'- -UCUGGUCggUCCUGUUauUGUGa-GCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 14382 | 0.68 | 0.672337 |
Target: 5'- -uACCGGCUuucGCAAUGGCGCUCuGCa -3' miRNA: 3'- ucUGGUCGGuccUGUUAUUGUGAG-CG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 7886 | 0.68 | 0.672337 |
Target: 5'- -uGCCaucaaGGCCAGuGACAAUGACG-UCGUa -3' miRNA: 3'- ucUGG-----UCGGUC-CUGUUAUUGUgAGCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 12774 | 0.67 | 0.695849 |
Target: 5'- -uACC-GCuCAGGACGcgAGCACagCGCa -3' miRNA: 3'- ucUGGuCG-GUCCUGUuaUUGUGa-GCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 5286 | 0.67 | 0.704016 |
Target: 5'- cGGCCAugaugaaauccaguGCCugacgcuGGACAAUGGCG-UCGCg -3' miRNA: 3'- uCUGGU--------------CGGu------CCUGUUAUUGUgAGCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 25290 | 0.67 | 0.712143 |
Target: 5'- cGGCgAGCCAGGGCGAUuuaaagguuauaaauGGCACa--- -3' miRNA: 3'- uCUGgUCGGUCCUGUUA---------------UUGUGagcg -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 5730 | 0.67 | 0.719073 |
Target: 5'- aGGuuuuCCAGCguGGuCAcgAACAC-CGCg -3' miRNA: 3'- -UCu---GGUCGguCCuGUuaUUGUGaGCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 22384 | 0.67 | 0.730542 |
Target: 5'- -cGCCAGCCGgauuuuuacGGACGGUcauuaguuAAC-CUCGCu -3' miRNA: 3'- ucUGGUCGGU---------CCUGUUA--------UUGuGAGCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 8887 | 0.67 | 0.741896 |
Target: 5'- -cGCCAGUCGGGAauCGGUGugGC-CGUu -3' miRNA: 3'- ucUGGUCGGUCCU--GUUAUugUGaGCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 23529 | 0.66 | 0.753121 |
Target: 5'- cGGCCAGCUucGcCAuguuGUGACGCUgCGCa -3' miRNA: 3'- uCUGGUCGGucCuGU----UAUUGUGA-GCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 834 | 0.66 | 0.7631 |
Target: 5'- uGGCCGGUgAGGcCAGUAGCuugauuaGCUgGCc -3' miRNA: 3'- uCUGGUCGgUCCuGUUAUUG-------UGAgCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 28230 | 0.66 | 0.784808 |
Target: 5'- uGACCcugaaacAGCCAGGGCGcucaGUGuugaAUUCGCc -3' miRNA: 3'- uCUGG-------UCGGUCCUGU----UAUug--UGAGCG- -5' |
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1288 | 5' | -51 | NC_001317.1 | + | 1038 | 0.66 | 0.785875 |
Target: 5'- -uGCCAGCCcGGAgAAgcUGACcggcgGCUCGUa -3' miRNA: 3'- ucUGGUCGGuCCUgUU--AUUG-----UGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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