Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1289 | 3' | -49 | NC_001317.1 | + | 1405 | 0.66 | 0.860456 |
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Target: 5'- gGUGUCUGGCGucagucggucacucuGGAUgcagCAuGCCAGCGc -3' miRNA: 3'- -CGUAGAUCGCu--------------CUUAaa--GU-CGGUCGC- -5' |
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| 1289 | 3' | -49 | NC_001317.1 | + | 21203 | 0.67 | 0.821142 |
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Target: 5'- cCAUCUGGgGcaAGuGUUgggCGGCCAGCa -3' miRNA: 3'- cGUAGAUCgC--UCuUAAa--GUCGGUCGc -5' |
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| 1289 | 3' | -49 | NC_001317.1 | + | 19260 | 0.68 | 0.789779 |
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Target: 5'- -uGUUUGGCGAccuGAAUaagcaGGCCAGCGa -3' miRNA: 3'- cgUAGAUCGCU---CUUAaag--UCGGUCGC- -5' |
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| 1289 | 3' | -49 | NC_001317.1 | + | 19503 | 0.68 | 0.756636 |
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Target: 5'- -uGUCaUGGUGAGAGaaaUCAGCCGGUc -3' miRNA: 3'- cgUAG-AUCGCUCUUaa-AGUCGGUCGc -5' |
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| 1289 | 3' | -49 | NC_001317.1 | + | 17729 | 0.7 | 0.686619 |
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Target: 5'- cCAUUUucGGCGAGGAggcCAGCuCGGCGg -3' miRNA: 3'- cGUAGA--UCGCUCUUaaaGUCG-GUCGC- -5' |
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| 1289 | 3' | -49 | NC_001317.1 | + | 4817 | 0.74 | 0.409839 |
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Target: 5'- aGCAUCgGGCGGGGguuccgcccgGUUUCGGUCuGCGg -3' miRNA: 3'- -CGUAGaUCGCUCU----------UAAAGUCGGuCGC- -5' |
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| 1289 | 3' | -49 | NC_001317.1 | + | 14681 | 0.92 | 0.031781 |
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Target: 5'- gGCAUaucGCGAGAAUUUCAGCCAGCGc -3' miRNA: 3'- -CGUAgauCGCUCUUAAAGUCGGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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