miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12894 3' -57.9 NC_003387.1 + 35698 0.66 0.525745
Target:  5'- gGCCG-CCUCGGCGGGcuccucGGGGc- -3'
miRNA:   3'- -UGGCuGGAGCCGCCCucuuu-CCUCuu -5'
12894 3' -57.9 NC_003387.1 + 22842 0.67 0.504882
Target:  5'- uGCCGACCUCGcgccguuucGCGagguauccgcaGGuGAGAGGGGGu -3'
miRNA:   3'- -UGGCUGGAGC---------CGC-----------CCuCUUUCCUCUu -5'
12894 3' -57.9 NC_003387.1 + 32273 0.67 0.504882
Target:  5'- gGCCGACaa-GGCGuGGGGGcgcGAGGAGc- -3'
miRNA:   3'- -UGGCUGgagCCGC-CCUCU---UUCCUCuu -5'
12894 3' -57.9 NC_003387.1 + 2053 0.69 0.35147
Target:  5'- aGCUGGCCgcggUGGCGGcgccauguucguguGAGAGGGGGGGg -3'
miRNA:   3'- -UGGCUGGa---GCCGCC--------------CUCUUUCCUCUu -5'
12894 3' -57.9 NC_003387.1 + 5959 1.06 0.000825
Target:  5'- gACCGACCUCGGCGGGAGAAAGGAGAAa -3'
miRNA:   3'- -UGGCUGGAGCCGCCCUCUUUCCUCUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.