Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
129 | 3' | -58.2 | AC_000006.1 | + | 26810 | 0.68 | 0.306172 |
Target: 5'- --aGCGGCUG-UGCUGCUGUcGGUa- -3' miRNA: 3'- gagCGCCGACgACGACGACGaCUAgc -5' |
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129 | 3' | -58.2 | AC_000006.1 | + | 32372 | 0.69 | 0.269087 |
Target: 5'- -cCGgGGCucggUGCgGCUGCUGCaaauggGAUCGg -3' miRNA: 3'- gaGCgCCG----ACGaCGACGACGa-----CUAGC- -5' |
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129 | 3' | -58.2 | AC_000006.1 | + | 30381 | 0.69 | 0.23571 |
Target: 5'- uUgGCGGCacGCUcCUGCUGCUGA-CGg -3' miRNA: 3'- gAgCGCCGa-CGAcGACGACGACUaGC- -5' |
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129 | 3' | -58.2 | AC_000006.1 | + | 11251 | 0.7 | 0.217398 |
Target: 5'- gUCGCug--GCUGCUGgaGCUGAUCa -3' miRNA: 3'- gAGCGccgaCGACGACgaCGACUAGc -5' |
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129 | 3' | -58.2 | AC_000006.1 | + | 3689 | 0.72 | 0.164953 |
Target: 5'- aUgGCGGCUGCgGCUGCcgcgGCUGcggCGg -3' miRNA: 3'- gAgCGCCGACGaCGACGa---CGACua-GC- -5' |
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129 | 3' | -58.2 | AC_000006.1 | + | 3742 | 0.72 | 0.151588 |
Target: 5'- --aGgGGCUaCUGCUGCUGCUGggCa -3' miRNA: 3'- gagCgCCGAcGACGACGACGACuaGc -5' |
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129 | 3' | -58.2 | AC_000006.1 | + | 12439 | 1.08 | 0.000267 |
Target: 5'- aCUCGCGGCUGCUGCUGCUGCUGAUCGc -3' miRNA: 3'- -GAGCGCCGACGACGACGACGACUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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