Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 129 | 5' | -54.9 | AC_000006.1 | + | 6496 | 0.66 | 0.575995 |
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Target: 5'- -gCGugAG-GGCggaGGCGUAcauGCCGCAAa -3' miRNA: 3'- ugGCugUCaCCG---UCGCAUu--UGGCGUU- -5' |
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| 129 | 5' | -54.9 | AC_000006.1 | + | 16767 | 0.66 | 0.57141 |
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Target: 5'- cGCCG-CAGgagaggcauggcaGGCAGCGgccUGAACCGCc- -3' miRNA: 3'- -UGGCuGUCa------------CCGUCGC---AUUUGGCGuu -5' |
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| 129 | 5' | -54.9 | AC_000006.1 | + | 29727 | 0.67 | 0.519456 |
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Target: 5'- gGCCcuaGACAGUcgGGCgaGGCGUGcgaaacuGCCGCAAa -3' miRNA: 3'- -UGG---CUGUCA--CCG--UCGCAUu------UGGCGUU- -5' |
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| 129 | 5' | -54.9 | AC_000006.1 | + | 15258 | 0.67 | 0.508398 |
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Target: 5'- -gCGGCGGUGGCAGUccgc-CCGCGc -3' miRNA: 3'- ugGCUGUCACCGUCGcauuuGGCGUu -5' |
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| 129 | 5' | -54.9 | AC_000006.1 | + | 21401 | 0.67 | 0.49744 |
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Target: 5'- cCCGAguucaAGUGGCAGCccaaGUAccaguACCGCAAc -3' miRNA: 3'- uGGCUg----UCACCGUCG----CAUu----UGGCGUU- -5' |
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| 129 | 5' | -54.9 | AC_000006.1 | + | 13107 | 0.67 | 0.465233 |
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Target: 5'- uGCCGggcucugugaACAGcGGCAGgGUGAGCCGg-- -3' miRNA: 3'- -UGGC----------UGUCaCCGUCgCAUUUGGCguu -5' |
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| 129 | 5' | -54.9 | AC_000006.1 | + | 10073 | 0.69 | 0.366576 |
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Target: 5'- uGCCGGCAGcGGUGGUGgaGGCgCGCGGg -3' miRNA: 3'- -UGGCUGUCaCCGUCGCauUUG-GCGUU- -5' |
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| 129 | 5' | -54.9 | AC_000006.1 | + | 15583 | 0.75 | 0.157825 |
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Target: 5'- gGCCG-CGGcGGCGGCGgcGGCCGCu- -3' miRNA: 3'- -UGGCuGUCaCCGUCGCauUUGGCGuu -5' |
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| 129 | 5' | -54.9 | AC_000006.1 | + | 12473 | 1.05 | 0.000886 |
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Target: 5'- cACCGACAGUGGCAGCGUAAACCGCAAc -3' miRNA: 3'- -UGGCUGUCACCGUCGCAUUUGGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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