miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
12903 3' -61.8 NC_003387.1 + 52676 0.66 0.378548
Target:  5'- -aGCGCGUUUgUgcaggucagcgaccCCUGG-GGGGGUGCc -3'
miRNA:   3'- ggCGCGCGAA-A--------------GGACCaCCCCCGCGc -5'
12903 3' -61.8 NC_003387.1 + 30752 0.67 0.317373
Target:  5'- cCCGCcgGUGCga-CCgGGUcGGGGGCGUu -3'
miRNA:   3'- -GGCG--CGCGaaaGGaCCA-CCCCCGCGc -5'
12903 3' -61.8 NC_003387.1 + 31193 0.67 0.317373
Target:  5'- -gGCgGCGCccaCCUGGcgcucGGGGGUGCGu -3'
miRNA:   3'- ggCG-CGCGaaaGGACCa----CCCCCGCGC- -5'
12903 3' -61.8 NC_003387.1 + 32262 0.67 0.317373
Target:  5'- gCCGuCGC-CgaggCCgacaagGcGUGGGGGCGCGa -3'
miRNA:   3'- -GGC-GCGcGaaa-GGa-----C-CACCCCCGCGC- -5'
12903 3' -61.8 NC_003387.1 + 50407 0.68 0.275078
Target:  5'- gCCGCGgGCgg-CCUGaaugaaGUcGGGGCGCa -3'
miRNA:   3'- -GGCGCgCGaaaGGAC------CAcCCCCGCGc -5'
12903 3' -61.8 NC_003387.1 + 46974 0.74 0.111374
Target:  5'- gCCGUGCacgacgaccucggGCUgcgCCgcaGGUGGGGGCGCc -3'
miRNA:   3'- -GGCGCG-------------CGAaa-GGa--CCACCCCCGCGc -5'
12903 3' -61.8 NC_003387.1 + 9218 1.1 0.000198
Target:  5'- cCCGCGCGCUUUCCUGGUGGGGGCGCGg -3'
miRNA:   3'- -GGCGCGCGAAAGGACCACCCCCGCGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.