Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12903 | 3' | -61.8 | NC_003387.1 | + | 52676 | 0.66 | 0.378548 |
Target: 5'- -aGCGCGUUUgUgcaggucagcgaccCCUGG-GGGGGUGCc -3' miRNA: 3'- ggCGCGCGAA-A--------------GGACCaCCCCCGCGc -5' |
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12903 | 3' | -61.8 | NC_003387.1 | + | 30752 | 0.67 | 0.317373 |
Target: 5'- cCCGCcgGUGCga-CCgGGUcGGGGGCGUu -3' miRNA: 3'- -GGCG--CGCGaaaGGaCCA-CCCCCGCGc -5' |
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12903 | 3' | -61.8 | NC_003387.1 | + | 31193 | 0.67 | 0.317373 |
Target: 5'- -gGCgGCGCccaCCUGGcgcucGGGGGUGCGu -3' miRNA: 3'- ggCG-CGCGaaaGGACCa----CCCCCGCGC- -5' |
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12903 | 3' | -61.8 | NC_003387.1 | + | 32262 | 0.67 | 0.317373 |
Target: 5'- gCCGuCGC-CgaggCCgacaagGcGUGGGGGCGCGa -3' miRNA: 3'- -GGC-GCGcGaaa-GGa-----C-CACCCCCGCGC- -5' |
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12903 | 3' | -61.8 | NC_003387.1 | + | 50407 | 0.68 | 0.275078 |
Target: 5'- gCCGCGgGCgg-CCUGaaugaaGUcGGGGCGCa -3' miRNA: 3'- -GGCGCgCGaaaGGAC------CAcCCCCGCGc -5' |
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12903 | 3' | -61.8 | NC_003387.1 | + | 46974 | 0.74 | 0.111374 |
Target: 5'- gCCGUGCacgacgaccucggGCUgcgCCgcaGGUGGGGGCGCc -3' miRNA: 3'- -GGCGCG-------------CGAaa-GGa--CCACCCCCGCGc -5' |
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12903 | 3' | -61.8 | NC_003387.1 | + | 9218 | 1.1 | 0.000198 |
Target: 5'- cCCGCGCGCUUUCCUGGUGGGGGCGCGg -3' miRNA: 3'- -GGCGCGCGAAAGGACCACCCCCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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