Results 41 - 49 of 49 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12904 | 5' | -62.1 | NC_003387.1 | + | 8181 | 0.67 | 0.338758 |
Target: 5'- aCGAGC-UGGGCUCGcccucgggcGGGUUGgCgCCGAc -3' miRNA: 3'- -GCUCGcGCCCGAGU---------UCCAGCgG-GGCU- -5' |
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12904 | 5' | -62.1 | NC_003387.1 | + | 7959 | 0.69 | 0.236202 |
Target: 5'- aCGAGUGCGGccgacCUCAAGGUCgGUgCCGu -3' miRNA: 3'- -GCUCGCGCCc----GAGUUCCAG-CGgGGCu -5' |
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12904 | 5' | -62.1 | NC_003387.1 | + | 7808 | 0.66 | 0.37106 |
Target: 5'- cCGAGCuGCaacgccuGGCggcCGAGGUCGCCCgGc -3' miRNA: 3'- -GCUCG-CGc------CCGa--GUUCCAGCGGGgCu -5' |
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12904 | 5' | -62.1 | NC_003387.1 | + | 6849 | 0.69 | 0.236202 |
Target: 5'- uGAGCGCGaccGCUCAGGG-CGCCaacaagacgCCGGu -3' miRNA: 3'- gCUCGCGCc--CGAGUUCCaGCGG---------GGCU- -5' |
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12904 | 5' | -62.1 | NC_003387.1 | + | 6792 | 0.69 | 0.226949 |
Target: 5'- cCGAGCGCGacauggucgcccugcGGCUCAAGGcgcgguUCGCCUa-- -3' miRNA: 3'- -GCUCGCGC---------------CCGAGUUCC------AGCGGGgcu -5' |
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12904 | 5' | -62.1 | NC_003387.1 | + | 4554 | 0.72 | 0.148799 |
Target: 5'- gCGGGCucggccuucgcgGCGGGCUCGGcGGUCGgCUCGGc -3' miRNA: 3'- -GCUCG------------CGCCCGAGUU-CCAGCgGGGCU- -5' |
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12904 | 5' | -62.1 | NC_003387.1 | + | 4510 | 0.66 | 0.379457 |
Target: 5'- -cGGCGCGGGCUCGGcGaaCGCCuuGc -3' miRNA: 3'- gcUCGCGCCCGAGUU-CcaGCGGggCu -5' |
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12904 | 5' | -62.1 | NC_003387.1 | + | 1111 | 0.75 | 0.091584 |
Target: 5'- gCGAGCGCGGGUUCGgccguGGGUucgccggugucgaCGUCCUGAu -3' miRNA: 3'- -GCUCGCGCCCGAGU-----UCCA-------------GCGGGGCU- -5' |
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12904 | 5' | -62.1 | NC_003387.1 | + | 178 | 0.66 | 0.396629 |
Target: 5'- gCGGGCGCGcaucaGCUCGGGGUCcaugugucuGCCUCc- -3' miRNA: 3'- -GCUCGCGCc----CGAGUUCCAG---------CGGGGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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