Results 1 - 3 of 3 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1291 | 5' | -56.9 | NC_001317.1 | + | 2732 | 0.69 | 0.275792 |
Target: 5'- gCCACUGGCCaucGUCG-CAGAGCUg--- -3' miRNA: 3'- aGGUGACCGG---CGGUaGUCUCGAgaua -5' |
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1291 | 5' | -56.9 | NC_001317.1 | + | 16333 | 1.05 | 0.000431 |
Target: 5'- uUCCACUGGCCGCCAUCAGAGCUCUAUu -3' miRNA: 3'- -AGGUGACCGGCGGUAGUCUCGAGAUA- -5' |
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1291 | 5' | -56.9 | NC_001317.1 | + | 19976 | 0.71 | 0.203998 |
Target: 5'- gCCGCUGGCCGUCAUCGcacuaccGGCUUUc- -3' miRNA: 3'- aGGUGACCGGCGGUAGUc------UCGAGAua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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