Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12910 | 3' | -60 | NC_003387.1 | + | 22343 | 0.66 | 0.463819 |
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Target: 5'- --cCGGcGCCGGUgGGgcUGG-CCCa -3' miRNA: 3'- aaaGCCcCGGCCAgCCauACCuGGGc -5' |
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| 12910 | 3' | -60 | NC_003387.1 | + | 22181 | 0.67 | 0.416656 |
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Target: 5'- --gCGGGGgCGG-CGGUaucggGUGGGCCa- -3' miRNA: 3'- aaaGCCCCgGCCaGCCA-----UACCUGGgc -5' |
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| 12910 | 3' | -60 | NC_003387.1 | + | 52161 | 0.68 | 0.355805 |
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Target: 5'- --gUGGGGCaggCGGUCGaGUA-GGGCCUGc -3' miRNA: 3'- aaaGCCCCG---GCCAGC-CAUaCCUGGGC- -5' |
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| 12910 | 3' | -60 | NC_003387.1 | + | 23860 | 0.68 | 0.355805 |
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Target: 5'- -gUCGGGGCCGuGgacCGGc---GACCCGg -3' miRNA: 3'- aaAGCCCCGGC-Ca--GCCauacCUGGGC- -5' |
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| 12910 | 3' | -60 | NC_003387.1 | + | 30491 | 0.69 | 0.308836 |
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Target: 5'- -gUCGGcGCCGGUCGa-GUGGACCg- -3' miRNA: 3'- aaAGCCcCGGCCAGCcaUACCUGGgc -5' |
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| 12910 | 3' | -60 | NC_003387.1 | + | 47471 | 0.69 | 0.293553 |
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Target: 5'- --gCGGGGCCGGUugccccgcgcuugCGGUGUcGGugUUGa -3' miRNA: 3'- aaaGCCCCGGCCA-------------GCCAUA-CCugGGC- -5' |
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| 12910 | 3' | -60 | NC_003387.1 | + | 23586 | 0.71 | 0.235321 |
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Target: 5'- aUUCGGGGUCGGcgCGGUuggccGGGCaCCa -3' miRNA: 3'- aAAGCCCCGGCCa-GCCAua---CCUG-GGc -5' |
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| 12910 | 3' | -60 | NC_003387.1 | + | 13765 | 1.07 | 0.000489 |
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Target: 5'- aUUUCGGGGCCGGUCGGUAUGGACCCGg -3' miRNA: 3'- -AAAGCCCCGGCCAGCCAUACCUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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