Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12913 | 5' | -50 | NC_003387.1 | + | 7559 | 0.67 | 0.843696 |
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Target: 5'- cAGCGGGUGCgGGUGCGc-GGCuCGGUg -3' miRNA: 3'- uUCGUUUACG-UCAUGUauCCGuGCCA- -5' |
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| 12913 | 5' | -50 | NC_003387.1 | + | 10911 | 0.67 | 0.843696 |
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Target: 5'- cGGCGAcgcgcUGCAGgACAUggucgcgcaGGGCGCGGUc -3' miRNA: 3'- uUCGUUu----ACGUCaUGUA---------UCCGUGCCA- -5' |
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| 12913 | 5' | -50 | NC_003387.1 | + | 27527 | 0.67 | 0.843696 |
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Target: 5'- cGGGCAGGUcgcacaucggGCAGUcagccagggcgACGUcGGCGCGGUg -3' miRNA: 3'- -UUCGUUUA----------CGUCA-----------UGUAuCCGUGCCA- -5' |
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| 12913 | 5' | -50 | NC_003387.1 | + | 47925 | 0.68 | 0.824622 |
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Target: 5'- uGGuCGGcgGCAG-GCAUGGGCACGa- -3' miRNA: 3'- uUC-GUUuaCGUCaUGUAUCCGUGCca -5' |
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| 12913 | 5' | -50 | NC_003387.1 | + | 5292 | 0.68 | 0.794304 |
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Target: 5'- cGAGCAGcaucGUGCGGUACccgAGGCGCc-- -3' miRNA: 3'- -UUCGUU----UACGUCAUGua-UCCGUGcca -5' |
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| 12913 | 5' | -50 | NC_003387.1 | + | 11200 | 0.68 | 0.794304 |
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Target: 5'- aGGGCAugucggacaaGAUGCAG-GCGUGGGCcgACGGc -3' miRNA: 3'- -UUCGU----------UUACGUCaUGUAUCCG--UGCCa -5' |
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| 12913 | 5' | -50 | NC_003387.1 | + | 22077 | 0.69 | 0.773102 |
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Target: 5'- uGGCGAcgGCGGUagcGCGUgGGGCACGu- -3' miRNA: 3'- uUCGUUuaCGUCA---UGUA-UCCGUGCca -5' |
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| 12913 | 5' | -50 | NC_003387.1 | + | 462 | 0.7 | 0.682741 |
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Target: 5'- -uGCGAGUGCAcucgACGagaUGGGCGCGGa -3' miRNA: 3'- uuCGUUUACGUca--UGU---AUCCGUGCCa -5' |
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| 12913 | 5' | -50 | NC_003387.1 | + | 49408 | 0.74 | 0.464612 |
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Target: 5'- cAGGCGGAUGCGG-AgGUGGGCGCGuGa -3' miRNA: 3'- -UUCGUUUACGUCaUgUAUCCGUGC-Ca -5' |
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| 12913 | 5' | -50 | NC_003387.1 | + | 15231 | 1.05 | 0.004498 |
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Target: 5'- cAAGCAAAUGCAGUACAUAGGCACGGUc -3' miRNA: 3'- -UUCGUUUACGUCAUGUAUCCGUGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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