Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1292 | 3' | -54.8 | NC_001317.1 | + | 27461 | 0.66 | 0.556712 |
Target: 5'- aAAAACugCGUCA--CCGACUgGCGCGa -3' miRNA: 3'- -UUUUGugGUGGUuaGGCUGGgCGCGC- -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 612 | 0.66 | 0.545399 |
Target: 5'- cAGAGCGCgACCGAcgUCGAaucgCUGCGCGa -3' miRNA: 3'- -UUUUGUGgUGGUUa-GGCUg---GGCGCGC- -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 16697 | 0.67 | 0.511942 |
Target: 5'- --uGCACgGCCAGcucaCCGGCCuCGCGUu -3' miRNA: 3'- uuuUGUGgUGGUUa---GGCUGG-GCGCGc -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 19628 | 0.67 | 0.502067 |
Target: 5'- aGAAGCGCCgaacccugccaGCCAAgccacaacgucagcaCCGcCCCGCGCa -3' miRNA: 3'- -UUUUGUGG-----------UGGUUa--------------GGCuGGGCGCGc -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 10505 | 0.68 | 0.458218 |
Target: 5'- --cGCAUCACCcgcgagCCGACCgGCGUc -3' miRNA: 3'- uuuUGUGGUGGuua---GGCUGGgCGCGc -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 6575 | 0.69 | 0.379043 |
Target: 5'- uAAACcuGCCACCAAaCCGGCagcaaaGCGCGg -3' miRNA: 3'- uUUUG--UGGUGGUUaGGCUGgg----CGCGC- -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 2434 | 0.69 | 0.379043 |
Target: 5'- uGGAGCGCCACCAGUggaagggcaUgGACuuugCCGCGCa -3' miRNA: 3'- -UUUUGUGGUGGUUA---------GgCUG----GGCGCGc -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 442 | 0.7 | 0.343045 |
Target: 5'- -cAGCGCCGCCGAcCUGAUggaCGCGCa -3' miRNA: 3'- uuUUGUGGUGGUUaGGCUGg--GCGCGc -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 15438 | 0.7 | 0.317704 |
Target: 5'- -uGACGCUGCCGAagCUGACCCGCaaGCu -3' miRNA: 3'- uuUUGUGGUGGUUa-GGCUGGGCG--CGc -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 9931 | 0.7 | 0.30797 |
Target: 5'- gGGAGC-CCACCAugugcgcgaaCGACCgGCGCGg -3' miRNA: 3'- -UUUUGuGGUGGUuag-------GCUGGgCGCGC- -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 14755 | 0.72 | 0.248212 |
Target: 5'- cGAACGCCACCGccacggcauacgccaCCG-CCCGCGCa -3' miRNA: 3'- uUUUGUGGUGGUua-------------GGCuGGGCGCGc -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 16154 | 0.75 | 0.155227 |
Target: 5'- ---cCGCCGCCAGaCUGACgCCGCGCa -3' miRNA: 3'- uuuuGUGGUGGUUaGGCUG-GGCGCGc -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 11030 | 0.76 | 0.138204 |
Target: 5'- -uGACACCACCAuccccCCGACCCcCGCu -3' miRNA: 3'- uuUUGUGGUGGUua---GGCUGGGcGCGc -5' |
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1292 | 3' | -54.8 | NC_001317.1 | + | 16673 | 1.06 | 0.000853 |
Target: 5'- aAAAACACCACCAAUCCGACCCGCGCGc -3' miRNA: 3'- -UUUUGUGGUGGUUAGGCUGGGCGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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