Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1292 | 5' | -56.2 | NC_001317.1 | + | 16707 | 1.1 | 0.000283 |
Target: 5'- aGGCACUUGAAGCGGCAAGGCGUGCCGc -3' miRNA: 3'- -CCGUGAACUUCGCCGUUCCGCACGGC- -5' |
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1292 | 5' | -56.2 | NC_001317.1 | + | 16623 | 0.71 | 0.229161 |
Target: 5'- aGGCGCUUGAuaaGGCGaaacGCGAGGcCGgugagcugGCCGu -3' miRNA: 3'- -CCGUGAACU---UCGC----CGUUCC-GCa-------CGGC- -5' |
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1292 | 5' | -56.2 | NC_001317.1 | + | 14515 | 0.69 | 0.299116 |
Target: 5'- aGGCGCUgcuaacugccGAAGCGGUcaccGGCGUuaaGCCGc -3' miRNA: 3'- -CCGUGAa---------CUUCGCCGuu--CCGCA---CGGC- -5' |
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1292 | 5' | -56.2 | NC_001317.1 | + | 6274 | 0.67 | 0.432849 |
Target: 5'- uGGCACUUGccGCGcaGCGccuGcGCGaUGCCGg -3' miRNA: 3'- -CCGUGAACuuCGC--CGUu--C-CGC-ACGGC- -5' |
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1292 | 5' | -56.2 | NC_001317.1 | + | 9406 | 0.76 | 0.112591 |
Target: 5'- uGGCGCUUucguGCGGCAuacGGgGUGCCGu -3' miRNA: 3'- -CCGUGAAcuu-CGCCGUu--CCgCACGGC- -5' |
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1292 | 5' | -56.2 | NC_001317.1 | + | 8608 | 0.66 | 0.47397 |
Target: 5'- cGGCAC-UGgcGCaGGCuguucGGGUGUGCa- -3' miRNA: 3'- -CCGUGaACuuCG-CCGu----UCCGCACGgc -5' |
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1292 | 5' | -56.2 | NC_001317.1 | + | 24081 | 0.66 | 0.495252 |
Target: 5'- cGG-ACUguGAGCGGCAcGG-GUGCCa -3' miRNA: 3'- -CCgUGAacUUCGCCGUuCCgCACGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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