Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12928 | 5' | -54.5 | NC_003387.1 | + | 36127 | 0.7 | 0.497944 |
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Target: 5'- cGGUUCGaGGUCGUCGAgUGAGCcccGACg -3' miRNA: 3'- aCUAAGC-CCAGCGGCU-GCUCGac-UUG- -5' |
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| 12928 | 5' | -54.5 | NC_003387.1 | + | 12571 | 0.7 | 0.497944 |
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Target: 5'- cGA-UCGGGcCGCCGACGgugucgccgugGGCcuUGAACu -3' miRNA: 3'- aCUaAGCCCaGCGGCUGC-----------UCG--ACUUG- -5' |
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| 12928 | 5' | -54.5 | NC_003387.1 | + | 45226 | 0.71 | 0.467122 |
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Target: 5'- cGAUcguggCGuGGUCggaGUCGAUGGGCUGGACg -3' miRNA: 3'- aCUAa----GC-CCAG---CGGCUGCUCGACUUG- -5' |
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| 12928 | 5' | -54.5 | NC_003387.1 | + | 3306 | 0.72 | 0.41806 |
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Target: 5'- cGAcUCGGG-CGCCGAgGuGCUGuGGCg -3' miRNA: 3'- aCUaAGCCCaGCGGCUgCuCGAC-UUG- -5' |
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| 12928 | 5' | -54.5 | NC_003387.1 | + | 48939 | 0.73 | 0.372265 |
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Target: 5'- ----gCGGGUUGCUGGCGGGC-GGGCu -3' miRNA: 3'- acuaaGCCCAGCGGCUGCUCGaCUUG- -5' |
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| 12928 | 5' | -54.5 | NC_003387.1 | + | 7158 | 0.73 | 0.372265 |
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Target: 5'- ---cUCGGccgUGCCGACGAGCUGGcgGCg -3' miRNA: 3'- acuaAGCCca-GCGGCUGCUCGACU--UG- -5' |
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| 12928 | 5' | -54.5 | NC_003387.1 | + | 33990 | 0.74 | 0.31412 |
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Target: 5'- cGggUCGcGGUCGaCGACGAGCUGccuGCu -3' miRNA: 3'- aCuaAGC-CCAGCgGCUGCUCGACu--UG- -5' |
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| 12928 | 5' | -54.5 | NC_003387.1 | + | 23381 | 1.1 | 0.000935 |
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Target: 5'- cUGAUUCGGGUCGCCGACGAGCUGAACa -3' miRNA: 3'- -ACUAAGCCCAGCGGCUGCUCGACUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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