Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1293 | 5' | -54.1 | NC_001317.1 | + | 28134 | 0.67 | 0.552789 |
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Target: 5'- cGCCGUUUCAgcaugGGCGGCGcGCU-GGCa- -3' miRNA: 3'- -CGGCGGAGU-----UCGCCGUuUGAaCUGgc -5' |
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| 1293 | 5' | -54.1 | NC_001317.1 | + | 9816 | 0.68 | 0.475563 |
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Target: 5'- uGCCGCgUacaAAGCGGCGggauccagacGACaUGGCUGa -3' miRNA: 3'- -CGGCGgAg--UUCGCCGU----------UUGaACUGGC- -5' |
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| 1293 | 5' | -54.1 | NC_001317.1 | + | 3503 | 0.7 | 0.394444 |
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Target: 5'- cCUG-CUCGGGCGGCAGAUUgaacGACUGg -3' miRNA: 3'- cGGCgGAGUUCGCCGUUUGAa---CUGGC- -5' |
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| 1293 | 5' | -54.1 | NC_001317.1 | + | 9296 | 0.72 | 0.275792 |
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Target: 5'- uGCCGCCggugaCAGGCuGGCAAuca-GACCGg -3' miRNA: 3'- -CGGCGGa----GUUCG-CCGUUugaaCUGGC- -5' |
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| 1293 | 5' | -54.1 | NC_001317.1 | + | 9922 | 0.72 | 0.268506 |
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Target: 5'- uGCUGCUggCAaucGGUGGCGAGCUUGAuaCCGc -3' miRNA: 3'- -CGGCGGa-GU---UCGCCGUUUGAACU--GGC- -5' |
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| 1293 | 5' | -54.1 | NC_001317.1 | + | 1637 | 0.74 | 0.215725 |
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Target: 5'- aCCGUCgUCGAGCuGCAGGCUgUGGCCGu -3' miRNA: 3'- cGGCGG-AGUUCGcCGUUUGA-ACUGGC- -5' |
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| 1293 | 5' | -54.1 | NC_001317.1 | + | 23566 | 0.75 | 0.182197 |
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Target: 5'- cGCCGCCUgAAuuGUGGCugcuGACUgaccUGACCGa -3' miRNA: 3'- -CGGCGGAgUU--CGCCGu---UUGA----ACUGGC- -5' |
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| 1293 | 5' | -54.1 | NC_001317.1 | + | 17775 | 1.12 | 0.000357 |
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Target: 5'- aGCCGCCUCAAGCGGCAAACUUGACCGg -3' miRNA: 3'- -CGGCGGAGUUCGCCGUUUGAACUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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