Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12935 | 3' | -52.6 | NC_003387.1 | + | 11561 | 0.66 | 0.867806 |
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Target: 5'- -aCCGgccGCAC--UCGCCCUgacaCUCGCa -3' miRNA: 3'- ggGGUa--UGUGaaAGCGGGAa---GAGCGc -5' |
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| 12935 | 3' | -52.6 | NC_003387.1 | + | 52712 | 0.66 | 0.867806 |
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Target: 5'- gCCCCGcgaacgauCGCgg-CGCCC--CUCGCGg -3' miRNA: 3'- -GGGGUau------GUGaaaGCGGGaaGAGCGC- -5' |
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| 12935 | 3' | -52.6 | NC_003387.1 | + | 3756 | 0.66 | 0.867806 |
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Target: 5'- gCCCgCGUGCACgaUCGCCagcUCaCGCa -3' miRNA: 3'- -GGG-GUAUGUGaaAGCGGga-AGaGCGc -5' |
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| 12935 | 3' | -52.6 | NC_003387.1 | + | 10502 | 0.66 | 0.859571 |
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Target: 5'- gCCCAUcgcgguGCGCcggUCGCCgUccugCUCGCGc -3' miRNA: 3'- gGGGUA------UGUGaa-AGCGGgAa---GAGCGC- -5' |
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| 12935 | 3' | -52.6 | NC_003387.1 | + | 23239 | 0.66 | 0.842378 |
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Target: 5'- gCCCAUcuCGCgggccgUGCCCUgCUCGCu -3' miRNA: 3'- gGGGUAu-GUGaaa---GCGGGAaGAGCGc -5' |
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| 12935 | 3' | -52.6 | NC_003387.1 | + | 196 | 0.68 | 0.775584 |
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Target: 5'- gCCgCCAgcucgGCgGCUUgUCGCUCUUgUCGCGg -3' miRNA: 3'- -GG-GGUa----UG-UGAA-AGCGGGAAgAGCGC- -5' |
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| 12935 | 3' | -52.6 | NC_003387.1 | + | 28175 | 0.68 | 0.754961 |
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Target: 5'- aCCCGgcgACAgUUUCGCUCggcccCUCGCc -3' miRNA: 3'- gGGGUa--UGUgAAAGCGGGaa---GAGCGc -5' |
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| 12935 | 3' | -52.6 | NC_003387.1 | + | 24635 | 0.69 | 0.712266 |
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Target: 5'- aCCCG-ACGCaccUCGCCCUgCUgCGCGa -3' miRNA: 3'- gGGGUaUGUGaa-AGCGGGAaGA-GCGC- -5' |
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| 12935 | 3' | -52.6 | NC_003387.1 | + | 6757 | 0.7 | 0.657159 |
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Target: 5'- gCCCAcagcGCACgccUCGCCCUUg-CGCGg -3' miRNA: 3'- gGGGUa---UGUGaa-AGCGGGAAgaGCGC- -5' |
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| 12935 | 3' | -52.6 | NC_003387.1 | + | 26997 | 1.11 | 0.001531 |
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Target: 5'- gCCCCAUACACUUUCGCCCUUCUCGCGu -3' miRNA: 3'- -GGGGUAUGUGAAAGCGGGAAGAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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