miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1294 3' -54.2 NC_001317.1 + 14811 0.66 0.586204
Target:  5'- aUCGACCAGACUaucgGCUgGCaCAAAACg-- -3'
miRNA:   3'- gAGCUGGUCUGA----CGG-CG-GUUUUGguu -5'
1294 3' -54.2 NC_001317.1 + 1353 0.67 0.517398
Target:  5'- -gUGACC-GACUGaCGCCAGAcACCAu -3'
miRNA:   3'- gaGCUGGuCUGACgGCGGUUU-UGGUu -5'
1294 3' -54.2 NC_001317.1 + 16325 0.67 0.469049
Target:  5'- --aGGCCGGGCgggaagucuauuCCGCCGAAGCCGGu -3'
miRNA:   3'- gagCUGGUCUGac----------GGCGGUUUUGGUU- -5'
1294 3' -54.2 NC_001317.1 + 11649 0.71 0.295033
Target:  5'- uCUUGAgguGGCUGCUGCCAGAGCCu- -3'
miRNA:   3'- -GAGCUgguCUGACGGCGGUUUUGGuu -5'
1294 3' -54.2 NC_001317.1 + 16174 0.71 0.287247
Target:  5'- aUCGACCucGGACUgcgccaccGCCGCCAGAcugacGCCGc -3'
miRNA:   3'- gAGCUGG--UCUGA--------CGGCGGUUU-----UGGUu -5'
1294 3' -54.2 NC_001317.1 + 6970 0.77 0.121858
Target:  5'- -gCG-CCAGACUGCCGCCGGuuuaagcaacuGGCCGAc -3'
miRNA:   3'- gaGCuGGUCUGACGGCGGUU-----------UUGGUU- -5'
1294 3' -54.2 NC_001317.1 + 18590 1.06 0.000811
Target:  5'- cCUCGACCAGACUGCCGCCAAAACCAAc -3'
miRNA:   3'- -GAGCUGGUCUGACGGCGGUUUUGGUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.