Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1294 | 3' | -54.2 | NC_001317.1 | + | 14811 | 0.66 | 0.586204 |
Target: 5'- aUCGACCAGACUaucgGCUgGCaCAAAACg-- -3' miRNA: 3'- gAGCUGGUCUGA----CGG-CG-GUUUUGguu -5' |
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1294 | 3' | -54.2 | NC_001317.1 | + | 1353 | 0.67 | 0.517398 |
Target: 5'- -gUGACC-GACUGaCGCCAGAcACCAu -3' miRNA: 3'- gaGCUGGuCUGACgGCGGUUU-UGGUu -5' |
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1294 | 3' | -54.2 | NC_001317.1 | + | 16325 | 0.67 | 0.469049 |
Target: 5'- --aGGCCGGGCgggaagucuauuCCGCCGAAGCCGGu -3' miRNA: 3'- gagCUGGUCUGac----------GGCGGUUUUGGUU- -5' |
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1294 | 3' | -54.2 | NC_001317.1 | + | 11649 | 0.71 | 0.295033 |
Target: 5'- uCUUGAgguGGCUGCUGCCAGAGCCu- -3' miRNA: 3'- -GAGCUgguCUGACGGCGGUUUUGGuu -5' |
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1294 | 3' | -54.2 | NC_001317.1 | + | 16174 | 0.71 | 0.287247 |
Target: 5'- aUCGACCucGGACUgcgccaccGCCGCCAGAcugacGCCGc -3' miRNA: 3'- gAGCUGG--UCUGA--------CGGCGGUUU-----UGGUu -5' |
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1294 | 3' | -54.2 | NC_001317.1 | + | 6970 | 0.77 | 0.121858 |
Target: 5'- -gCG-CCAGACUGCCGCCGGuuuaagcaacuGGCCGAc -3' miRNA: 3'- gaGCuGGUCUGACGGCGGUU-----------UUGGUU- -5' |
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1294 | 3' | -54.2 | NC_001317.1 | + | 18590 | 1.06 | 0.000811 |
Target: 5'- cCUCGACCAGACUGCCGCCAAAACCAAc -3' miRNA: 3'- -GAGCUGGUCUGACGGCGGUUUUGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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