Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1294 | 5' | -54.1 | NC_001317.1 | + | 3621 | 0.68 | 0.45692 |
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Target: 5'- aCCUgcaGCAUUUCGGCAAUCugcGGCACGg- -3' miRNA: 3'- aGGA---UGUAGGGCCGUUGG---UCGUGUau -5' |
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| 1294 | 5' | -54.1 | NC_001317.1 | + | 14621 | 0.68 | 0.414591 |
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Target: 5'- uUCCUGCGUaucgagaCCCGGCAcACCGagaauGCGCGg- -3' miRNA: 3'- -AGGAUGUA-------GGGCCGU-UGGU-----CGUGUau -5' |
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| 1294 | 5' | -54.1 | NC_001317.1 | + | 17159 | 0.68 | 0.412588 |
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Target: 5'- gCCUGCAuUUCUGGCGACUgcuuaucgaccccgAGCACAg- -3' miRNA: 3'- aGGAUGU-AGGGCCGUUGG--------------UCGUGUau -5' |
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| 1294 | 5' | -54.1 | NC_001317.1 | + | 26307 | 0.69 | 0.386146 |
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Target: 5'- aUCUGCAUCCUccucccacauaGGUAACgCAGCGCGg- -3' miRNA: 3'- aGGAUGUAGGG-----------CCGUUG-GUCGUGUau -5' |
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| 1294 | 5' | -54.1 | NC_001317.1 | + | 6164 | 0.69 | 0.371014 |
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Target: 5'- aUCCUGCgcaccacgaccaucaGUCagcacaCCGGCGACCcacGGCGCAUAg -3' miRNA: 3'- -AGGAUG---------------UAG------GGCCGUUGG---UCGUGUAU- -5' |
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| 1294 | 5' | -54.1 | NC_001317.1 | + | 7239 | 0.71 | 0.275262 |
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Target: 5'- gUCCUGCAga-CGcGCAacugACCAGCGCAUAu -3' miRNA: 3'- -AGGAUGUaggGC-CGU----UGGUCGUGUAU- -5' |
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| 1294 | 5' | -54.1 | NC_001317.1 | + | 18635 | 1.07 | 0.000649 |
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Target: 5'- uUCCUACAUCCCGGCAACCAGCACAUAu -3' miRNA: 3'- -AGGAUGUAGGGCCGUUGGUCGUGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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