Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1295 | 3' | -54.5 | NC_001317.1 | + | 605 | 0.66 | 0.591826 |
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Target: 5'- cGACcGACGucgaaucGCUGCGCGAgGCGaugcgcaaCUCGAa -3' miRNA: 3'- -CUGuCUGU-------CGAUGCGCUgUGC--------GAGCU- -5' |
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| 1295 | 3' | -54.5 | NC_001317.1 | + | 29784 | 0.66 | 0.581492 |
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Target: 5'- aGCGGAacCGGCUACGCca-ACGUUUGAg -3' miRNA: 3'- cUGUCU--GUCGAUGCGcugUGCGAGCU- -5' |
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| 1295 | 3' | -54.5 | NC_001317.1 | + | 20284 | 0.66 | 0.558672 |
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Target: 5'- aGugGGAUaAGCUGCaGCGAgGCGUUgCGGa -3' miRNA: 3'- -CugUCUG-UCGAUG-CGCUgUGCGA-GCU- -5' |
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| 1295 | 3' | -54.5 | NC_001317.1 | + | 12696 | 0.67 | 0.526062 |
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Target: 5'- aACAGACAGCgcgucugaggugcuUGCGGCgACGC-CGAa -3' miRNA: 3'- cUGUCUGUCGau------------GCGCUG-UGCGaGCU- -5' |
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| 1295 | 3' | -54.5 | NC_001317.1 | + | 24753 | 0.68 | 0.460091 |
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Target: 5'- uGGC-GACGGCUGCGCgcguugcuuauGugGCGUUCGc -3' miRNA: 3'- -CUGuCUGUCGAUGCG-----------CugUGCGAGCu -5' |
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| 1295 | 3' | -54.5 | NC_001317.1 | + | 6328 | 0.69 | 0.379834 |
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Target: 5'- cGCAGGC-GCUGCGCGGCAagUGCcacuuccUCGAc -3' miRNA: 3'- cUGUCUGuCGAUGCGCUGU--GCG-------AGCU- -5' |
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| 1295 | 3' | -54.5 | NC_001317.1 | + | 6448 | 0.73 | 0.238243 |
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Target: 5'- cGCAGuuuCAGC-GCGCGAUGCaGCUCGAc -3' miRNA: 3'- cUGUCu--GUCGaUGCGCUGUG-CGAGCU- -5' |
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| 1295 | 3' | -54.5 | NC_001317.1 | + | 18779 | 1.09 | 0.000555 |
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Target: 5'- uGACAGACAGCUACGCGACACGCUCGAa -3' miRNA: 3'- -CUGUCUGUCGAUGCGCUGUGCGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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