Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1295 | 5' | -54.1 | NC_001317.1 | + | 14544 | 0.66 | 0.633438 |
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Target: 5'- gGCGuuAAGCc-GCGCauucUCGGUGUGCc -3' miRNA: 3'- gCGCuuUUCGcaCGCGc---AGUCGCACG- -5' |
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| 1295 | 5' | -54.1 | NC_001317.1 | + | 9802 | 0.66 | 0.633438 |
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Target: 5'- cCGUGcAGGGGCuGUGcCGCGUacaaAGCG-GCg -3' miRNA: 3'- -GCGC-UUUUCG-CAC-GCGCAg---UCGCaCG- -5' |
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| 1295 | 5' | -54.1 | NC_001317.1 | + | 17208 | 0.68 | 0.508679 |
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Target: 5'- uGCGAAAAGCG-GCGgUGaugCAGC-UGCc -3' miRNA: 3'- gCGCUUUUCGCaCGC-GCa--GUCGcACG- -5' |
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| 1295 | 5' | -54.1 | NC_001317.1 | + | 9120 | 0.68 | 0.497774 |
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Target: 5'- -uUGAcGAGCGUGCGC-UCGGUGaGCc -3' miRNA: 3'- gcGCUuUUCGCACGCGcAGUCGCaCG- -5' |
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| 1295 | 5' | -54.1 | NC_001317.1 | + | 15510 | 0.68 | 0.476289 |
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Target: 5'- cCGCGAuaguuuucGAGCuUGCGgGUCAGCuucgGCa -3' miRNA: 3'- -GCGCUu-------UUCGcACGCgCAGUCGca--CG- -5' |
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| 1295 | 5' | -54.1 | NC_001317.1 | + | 16891 | 0.7 | 0.358453 |
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Target: 5'- aCGUGAGGcacuGGC--GCGCGUCAGUGcGCa -3' miRNA: 3'- -GCGCUUU----UCGcaCGCGCAGUCGCaCG- -5' |
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| 1295 | 5' | -54.1 | NC_001317.1 | + | 2001 | 0.73 | 0.255614 |
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Target: 5'- gGCGGAAAGCG-GUuCGUUuaacagGGCGUGCa -3' miRNA: 3'- gCGCUUUUCGCaCGcGCAG------UCGCACG- -5' |
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| 1295 | 5' | -54.1 | NC_001317.1 | + | 18815 | 1.11 | 0.000446 |
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Target: 5'- gCGCGAAAAGCGUGCGCGUCAGCGUGCc -3' miRNA: 3'- -GCGCUUUUCGCACGCGCAGUCGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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