Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12951 | 3' | -58.6 | NC_003387.1 | + | 39233 | 0.73 | 0.215585 |
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Target: 5'- cGAaCCCGCCGGGGcugucgGUGGUgcccuccucgccgucGCGGCCc -3' miRNA: 3'- uCUaGGGCGGCUCCa-----CACCG---------------UGCUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 49345 | 0.74 | 0.187351 |
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Target: 5'- gGGAUgCCCGCCGAGGccagcuUGGUgaugccgucgACGACCg -3' miRNA: 3'- -UCUA-GGGCGGCUCCac----ACCG----------UGCUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 1519 | 0.75 | 0.151521 |
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Target: 5'- cGGUCgugaaGUCGGGGcgGUGGCACGACCu -3' miRNA: 3'- uCUAGgg---CGGCUCCa-CACCGUGCUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 41751 | 0.76 | 0.128856 |
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Target: 5'- cGG-CUgGCCGcGGUGUGGUGCGACCu -3' miRNA: 3'- uCUaGGgCGGCuCCACACCGUGCUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 35627 | 0.77 | 0.109361 |
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Target: 5'- aGGAgCCCGCCGAGGcggccgGgcgGGCACuGGCCg -3' miRNA: 3'- -UCUaGGGCGGCUCCa-----Ca--CCGUG-CUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 33601 | 1.11 | 0.000361 |
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Target: 5'- aAGAUCCCGCCGAGGUGUGGCACGACCu -3' miRNA: 3'- -UCUAGGGCGGCUCCACACCGUGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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