Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12951 | 3' | -58.6 | NC_003387.1 | + | 6827 | 0.7 | 0.340545 |
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Target: 5'- cGGUUCgccuacgUGCUG-GGUGUGaGCGCGACCg -3' miRNA: 3'- uCUAGG-------GCGGCuCCACAC-CGUGCUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 4061 | 0.69 | 0.37449 |
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Target: 5'- cGAUUCCGaggUGGuGGUGUGGUugGACa -3' miRNA: 3'- uCUAGGGCg--GCU-CCACACCGugCUGg -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 2416 | 0.68 | 0.437507 |
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Target: 5'- cGAcCCCGCCuGGGagaUGUGGCAgCGcaACCg -3' miRNA: 3'- uCUaGGGCGGcUCC---ACACCGU-GC--UGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 1948 | 0.67 | 0.466315 |
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Target: 5'- uGAUCCgGCCGcGGGUcUGGC-CGGgCa -3' miRNA: 3'- uCUAGGgCGGC-UCCAcACCGuGCUgG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 1519 | 0.75 | 0.151521 |
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Target: 5'- cGGUCgugaaGUCGGGGcgGUGGCACGACCu -3' miRNA: 3'- uCUAGgg---CGGCUCCa-CACCGUGCUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 1267 | 0.66 | 0.526664 |
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Target: 5'- cGGAcCCCGgCGAGGUGUucacGGUgauGCG-CCu -3' miRNA: 3'- -UCUaGGGCgGCUCCACA----CCG---UGCuGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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