Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12951 | 3' | -58.6 | NC_003387.1 | + | 4061 | 0.69 | 0.37449 |
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Target: 5'- cGAUUCCGaggUGGuGGUGUGGUugGACa -3' miRNA: 3'- uCUAGGGCg--GCU-CCACACCGugCUGg -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 51465 | 0.7 | 0.341346 |
|
Target: 5'- ---cCUCGCCGGGGUccgucggcuucgGUGGCGuacCGGCCa -3' miRNA: 3'- ucuaGGGCGGCUCCA------------CACCGU---GCUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 27145 | 0.72 | 0.242441 |
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Target: 5'- ---gCCCGCCGGGGUc-GGCGCcGCCg -3' miRNA: 3'- ucuaGGGCGGCUCCAcaCCGUGcUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 39233 | 0.73 | 0.215585 |
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Target: 5'- cGAaCCCGCCGGGGcugucgGUGGUgcccuccucgccgucGCGGCCc -3' miRNA: 3'- uCUaGGGCGGCUCCa-----CACCG---------------UGCUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 49345 | 0.74 | 0.187351 |
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Target: 5'- gGGAUgCCCGCCGAGGccagcuUGGUgaugccgucgACGACCg -3' miRNA: 3'- -UCUA-GGGCGGCUCCac----ACCG----------UGCUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 42046 | 0.66 | 0.568477 |
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Target: 5'- ----aCCGCCGAGGcggccGUGGuCGCaGCCg -3' miRNA: 3'- ucuagGGCGGCUCCa----CACC-GUGcUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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