Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12951 | 3' | -58.6 | NC_003387.1 | + | 48194 | 0.69 | 0.360974 |
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Target: 5'- aAGGUgCCCgcaccggcggcggcgGCCGAGGUGacccGCACGAUCg -3' miRNA: 3'- -UCUA-GGG---------------CGGCUCCACac--CGUGCUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 36005 | 0.69 | 0.37449 |
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Target: 5'- cGAUCgaCGgCGAGGUcGUGGUGCG-CCg -3' miRNA: 3'- uCUAGg-GCgGCUCCA-CACCGUGCuGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 48820 | 0.69 | 0.37449 |
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Target: 5'- -cGUCCCGCCGGucGUGcugcucGGCGCGACg -3' miRNA: 3'- ucUAGGGCGGCUc-CACa-----CCGUGCUGg -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 31011 | 0.69 | 0.39186 |
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Target: 5'- aGGAUCggGCCGAGcGUGcgccggGGCGgCGGCCa -3' miRNA: 3'- -UCUAGggCGGCUC-CACa-----CCGU-GCUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 11979 | 0.69 | 0.39186 |
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Target: 5'- cGAUCUUccauuucaUCGAGGacgUGUGGCGCGGCCu -3' miRNA: 3'- uCUAGGGc-------GGCUCC---ACACCGUGCUGG- -5' |
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| 12951 | 3' | -58.6 | NC_003387.1 | + | 33601 | 1.11 | 0.000361 |
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Target: 5'- aAGAUCCCGCCGAGGUGUGGCACGACCu -3' miRNA: 3'- -UCUAGGGCGGCUCCACACCGUGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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