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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1296 | 3' | -46.7 | NC_001317.1 | + | 27081 | 1.08 | 0.003935 |
Target: 5'- gCAUGGUCAAUAUGGUCGCCAGAUUUAa -3' miRNA: 3'- -GUACCAGUUAUACCAGCGGUCUAAAU- -5' |
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1296 | 3' | -46.7 | NC_001317.1 | + | 17099 | 0.7 | 0.796704 |
Target: 5'- --gGGUCGAUAagcaGUCGCCAGAa--- -3' miRNA: 3'- guaCCAGUUAUac--CAGCGGUCUaaau -5' |
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1296 | 3' | -46.7 | NC_001317.1 | + | 7568 | 0.67 | 0.92876 |
Target: 5'- gGUGGUUuuguuucaAUGGUCGCCGGuGUUg- -3' miRNA: 3'- gUACCAGuua-----UACCAGCGGUC-UAAau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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