miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1297 3' -50 NC_001317.1 + 15962 0.66 0.844557
Target:  5'- uGCUCUGAauggggguaaUAACCGGCcagCAgugcugacCGGUCAGg -3'
miRNA:   3'- -UGAGACU----------GUUGGCCGua-GU--------GUUAGUC- -5'
1297 3' -50 NC_001317.1 + 6350 0.66 0.834982
Target:  5'- -gUC-GACAgacucACCGGCAUCGCg--CAGg -3'
miRNA:   3'- ugAGaCUGU-----UGGCCGUAGUGuuaGUC- -5'
1297 3' -50 NC_001317.1 + 18442 0.66 0.815084
Target:  5'- cGCUC-GAUAGCugcaaaaauguCGGCGUCAUAuuugGUCAGg -3'
miRNA:   3'- -UGAGaCUGUUG-----------GCCGUAGUGU----UAGUC- -5'
1297 3' -50 NC_001317.1 + 19849 0.66 0.804785
Target:  5'- uUUCUGACccGACUGGCugacCGCAAUgGGg -3'
miRNA:   3'- uGAGACUG--UUGGCCGua--GUGUUAgUC- -5'
1297 3' -50 NC_001317.1 + 6538 0.66 0.804785
Target:  5'- cGCUCaaucuccuUGcGCAcACCGGCAUUGCGGUCGa -3'
miRNA:   3'- -UGAG--------AC-UGU-UGGCCGUAGUGUUAGUc -5'
1297 3' -50 NC_001317.1 + 7784 0.67 0.761578
Target:  5'- uAUUCUGGugaguguuaaUGACCGGCAUCACuuUCc- -3'
miRNA:   3'- -UGAGACU----------GUUGGCCGUAGUGuuAGuc -5'
1297 3' -50 NC_001317.1 + 6471 0.77 0.237671
Target:  5'- cCUCUG-CAuucauACCGGCAUCACGcaggGUCAGg -3'
miRNA:   3'- uGAGACuGU-----UGGCCGUAGUGU----UAGUC- -5'
1297 3' -50 NC_001317.1 + 28738 1.08 0.001723
Target:  5'- cACUCUGACAACCGGCAUCACAAUCAGc -3'
miRNA:   3'- -UGAGACUGUUGGCCGUAGUGUUAGUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.