miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1298 5' -56.3 NC_001331.1 + 274 1.1 0.000043
Target:  5'- cCGACCGAAACGGUCUUGGGUCGGGCGg -3'
miRNA:   3'- -GCUGGCUUUGCCAGAACCCAGCCCGC- -5'
1298 5' -56.3 NC_001331.1 + 388 0.76 0.030739
Target:  5'- gGGCCGGAcGCGGggCUUGGGccuggcuugCGGGCGg -3'
miRNA:   3'- gCUGGCUU-UGCCa-GAACCCa--------GCCCGC- -5'
1298 5' -56.3 NC_001331.1 + 107 0.68 0.118746
Target:  5'- gGACCGAGGCguagauuuccccGGUCUU-GGUCGaGGUa -3'
miRNA:   3'- gCUGGCUUUG------------CCAGAAcCCAGC-CCGc -5'
1298 5' -56.3 NC_001331.1 + 6890 0.67 0.153011
Target:  5'- uGACCGGAuCGGcCac-GGUgCGGGCGg -3'
miRNA:   3'- gCUGGCUUuGCCaGaacCCA-GCCCGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.