Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12985 | 5' | -58.6 | NC_003387.1 | + | 35429 | 0.66 | 0.486257 |
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Target: 5'- uUGGCgGAUUcGCCGGCUGUuagcugucGGGGUc -3' miRNA: 3'- cACCGaCUAGcUGGCCGACGu-------CCUCA- -5' |
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| 12985 | 5' | -58.6 | NC_003387.1 | + | 18183 | 0.66 | 0.46614 |
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Target: 5'- --cGCUGA-CGAUCGGCUGCgAGGuGc -3' miRNA: 3'- cacCGACUaGCUGGCCGACG-UCCuCa -5' |
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| 12985 | 5' | -58.6 | NC_003387.1 | + | 12010 | 0.67 | 0.444521 |
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Target: 5'- -cGGcCUGGUCGACaCGGCgcaugcggugugGCAGGGc- -3' miRNA: 3'- caCC-GACUAGCUG-GCCGa-----------CGUCCUca -5' |
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| 12985 | 5' | -58.6 | NC_003387.1 | + | 1279 | 0.67 | 0.436799 |
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Target: 5'- -cGGCUGcgcggcguUCGACCGGC-GCAGuucGAGg -3' miRNA: 3'- caCCGACu-------AGCUGGCCGaCGUC---CUCa -5' |
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| 12985 | 5' | -58.6 | NC_003387.1 | + | 20342 | 0.67 | 0.427257 |
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Target: 5'- uUGGUUGuGUCGGCCaGCccccgccucgUGCGGGGGUu -3' miRNA: 3'- cACCGAC-UAGCUGGcCG----------ACGUCCUCA- -5' |
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| 12985 | 5' | -58.6 | NC_003387.1 | + | 27858 | 0.67 | 0.40855 |
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Target: 5'- -cGGCgccacGAUCGAcaCCGGCgcggucgaGCAGGAGc -3' miRNA: 3'- caCCGa----CUAGCU--GGCCGa-------CGUCCUCa -5' |
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| 12985 | 5' | -58.6 | NC_003387.1 | + | 22547 | 0.68 | 0.38147 |
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Target: 5'- -gGGCUGGcUCGACgCGGUggcGCAGGcAGa -3' miRNA: 3'- caCCGACU-AGCUG-GCCGa--CGUCC-UCa -5' |
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| 12985 | 5' | -58.6 | NC_003387.1 | + | 4896 | 0.69 | 0.307693 |
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Target: 5'- -aGGCUGAUCuGCCGGgUGCGGuAGc -3' miRNA: 3'- caCCGACUAGcUGGCCgACGUCcUCa -5' |
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| 12985 | 5' | -58.6 | NC_003387.1 | + | 24959 | 0.69 | 0.307693 |
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Target: 5'- aUGGCcugGGUCGGuuGGCagcucggcaUGCAGGGGg -3' miRNA: 3'- cACCGa--CUAGCUggCCG---------ACGUCCUCa -5' |
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| 12985 | 5' | -58.6 | NC_003387.1 | + | 45602 | 1.06 | 0.000584 |
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Target: 5'- cGUGGCUGAUCGACCGGCUGCAGGAGUc -3' miRNA: 3'- -CACCGACUAGCUGGCCGACGUCCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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