Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
12988 | 3' | -52.3 | NC_003387.1 | + | 3993 | 0.66 | 0.85321 |
Target: 5'- aGGAUUGAGGcggccGUGGcGGCGaucaGCCAGGc -3' miRNA: 3'- -CCUAGCUCUu----CACU-UCGCg---UGGUCCu -5' |
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12988 | 3' | -52.3 | NC_003387.1 | + | 22371 | 0.66 | 0.835523 |
Target: 5'- cGGUCGAcucGUGAAuguggccgccGgGCACCAGGAa -3' miRNA: 3'- cCUAGCUcuuCACUU----------CgCGUGGUCCU- -5' |
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12988 | 3' | -52.3 | NC_003387.1 | + | 42168 | 0.67 | 0.807346 |
Target: 5'- cGAUCGAGGAGcUGAccgAGCGCcucaACgAGGc -3' miRNA: 3'- cCUAGCUCUUC-ACU---UCGCG----UGgUCCu -5' |
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12988 | 3' | -52.3 | NC_003387.1 | + | 39217 | 0.67 | 0.797556 |
Target: 5'- cGGUCGGGcAGUgGGAGCggguaccgGCGCCGGGc -3' miRNA: 3'- cCUAGCUCuUCA-CUUCG--------CGUGGUCCu -5' |
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12988 | 3' | -52.3 | NC_003387.1 | + | 731 | 0.69 | 0.724637 |
Target: 5'- aGGUCGGGc----AGGUGCGCCAGGAu -3' miRNA: 3'- cCUAGCUCuucacUUCGCGUGGUCCU- -5' |
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12988 | 3' | -52.3 | NC_003387.1 | + | 27131 | 0.69 | 0.679471 |
Target: 5'- cGGGUCGAGGccgugcccgccggGGUc-GGCGCcGCCGGGGa -3' miRNA: 3'- -CCUAGCUCU-------------UCAcuUCGCG-UGGUCCU- -5' |
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12988 | 3' | -52.3 | NC_003387.1 | + | 14336 | 0.7 | 0.635761 |
Target: 5'- aGGUCGAGGucGUGca--GCACCAGGAu -3' miRNA: 3'- cCUAGCUCUu-CACuucgCGUGGUCCU- -5' |
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12988 | 3' | -52.3 | NC_003387.1 | + | 31011 | 0.71 | 0.579719 |
Target: 5'- aGGAUCGGGcc--GAGcGUGCGCCGGGGc -3' miRNA: 3'- -CCUAGCUCuucaCUU-CGCGUGGUCCU- -5' |
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12988 | 3' | -52.3 | NC_003387.1 | + | 43639 | 0.73 | 0.471954 |
Target: 5'- cGGGUCGAGGGacu-GGCGCACCAGc- -3' miRNA: 3'- -CCUAGCUCUUcacuUCGCGUGGUCcu -5' |
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12988 | 3' | -52.3 | NC_003387.1 | + | 16026 | 0.73 | 0.468868 |
Target: 5'- cGGGUCGAGAuucagcuugcccgcGGUGAA-CGCgGCCAGGu -3' miRNA: 3'- -CCUAGCUCU--------------UCACUUcGCG-UGGUCCu -5' |
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12988 | 3' | -52.3 | NC_003387.1 | + | 50248 | 0.77 | 0.278194 |
Target: 5'- cGGAUCGAgGAGGUGGccgacguGCGCACCgAGGu -3' miRNA: 3'- -CCUAGCU-CUUCACUu------CGCGUGG-UCCu -5' |
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12988 | 3' | -52.3 | NC_003387.1 | + | 46847 | 1.12 | 0.00129 |
Target: 5'- cGGAUCGAGAAGUGAAGCGCACCAGGAc -3' miRNA: 3'- -CCUAGCUCUUCACUUCGCGUGGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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