miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1299 3' -50.4 NC_001331.1 + 778 1.1 0.000168
Target:  5'- aAUCAAAAGCCCCGUCACGAACGAUCCc -3'
miRNA:   3'- -UAGUUUUCGGGGCAGUGCUUGCUAGG- -5'
1299 3' -50.4 NC_001331.1 + 1810 0.67 0.3515
Target:  5'- -gCAGAAG-CCCGUCGUGGugGAgCCu -3'
miRNA:   3'- uaGUUUUCgGGGCAGUGCUugCUaGG- -5'
1299 3' -50.4 NC_001331.1 + 2216 0.66 0.372155
Target:  5'- -cCGAAGGCCCCGgcgGCGAACu---- -3'
miRNA:   3'- uaGUUUUCGGGGCag-UGCUUGcuagg -5'
1299 3' -50.4 NC_001331.1 + 3633 0.66 0.372155
Target:  5'- ---uGGGGCCuCUGcgCACGAuugGCGGUCCg -3'
miRNA:   3'- uaguUUUCGG-GGCa-GUGCU---UGCUAGG- -5'
1299 3' -50.4 NC_001331.1 + 4618 0.66 0.404651
Target:  5'- gGUCGAGuuCgCCGgcUACGAAUGGUCCa -3'
miRNA:   3'- -UAGUUUucGgGGCa-GUGCUUGCUAGG- -5'
1299 3' -50.4 NC_001331.1 + 6280 0.66 0.403539
Target:  5'- gAUCGAcuuGCCCCGgucgaggUCACGGcggauGCGAUgCa -3'
miRNA:   3'- -UAGUUuu-CGGGGC-------AGUGCU-----UGCUAgG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.