Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12996 | 5' | -53.2 | NC_003387.1 | + | 19450 | 0.66 | 0.797556 |
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Target: 5'- aGGCcgugCGGGcGCGUgucaGUGUgGGCGCCg -3' miRNA: 3'- -CUGa---GCCC-CGCAaa--CACAaCUGUGGa -5' |
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| 12996 | 5' | -53.2 | NC_003387.1 | + | 29210 | 0.66 | 0.777446 |
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Target: 5'- cGAUUCGGGGuCGgcgcggUUGgccgGGCACCa -3' miRNA: 3'- -CUGAGCCCC-GCa-----AACacaaCUGUGGa -5' |
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| 12996 | 5' | -53.2 | NC_003387.1 | + | 44624 | 0.68 | 0.669423 |
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Target: 5'- gGGCUCGcccucGGGCGg----GUUGGCGCCg -3' miRNA: 3'- -CUGAGC-----CCCGCaaacaCAACUGUGGa -5' |
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| 12996 | 5' | -53.2 | NC_003387.1 | + | 5318 | 0.7 | 0.579719 |
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Target: 5'- gGGCUCGGGGCGg--GgccgGUUGccccGCGCUUg -3' miRNA: 3'- -CUGAGCCCCGCaaaCa---CAAC----UGUGGA- -5' |
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| 12996 | 5' | -53.2 | NC_003387.1 | + | 27375 | 0.71 | 0.512958 |
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Target: 5'- aGACUCGGugcgggcguucacGGCGUUcucUGUGUacgacgcgcgcUGGCACCa -3' miRNA: 3'- -CUGAGCC-------------CCGCAA---ACACA-----------ACUGUGGa -5' |
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| 12996 | 5' | -53.2 | NC_003387.1 | + | 3306 | 0.72 | 0.451578 |
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Target: 5'- cGACUCGGGcGCcgagGUgcUGUGgcgUGACACCg -3' miRNA: 3'- -CUGAGCCC-CG----CAa-ACACa--ACUGUGGa -5' |
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| 12996 | 5' | -53.2 | NC_003387.1 | + | 50550 | 0.89 | 0.037656 |
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Target: 5'- uGGCUCggGGGGCGcUUUGUGUUGACACCUc -3' miRNA: 3'- -CUGAG--CCCCGC-AAACACAACUGUGGA- -5' |
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| 12996 | 5' | -53.2 | NC_003387.1 | + | 49963 | 1.07 | 0.00204 |
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Target: 5'- gGACUCGGGGCGUUUGUGUUGACACCUc -3' miRNA: 3'- -CUGAGCCCCGCAAACACAACUGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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