Results 21 - 31 of 31 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12998 | 3' | -48.8 | NC_003387.1 | + | 36200 | 0.71 | 0.79026 |
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Target: 5'- cGCGCUgc-UG-UGGCGCCUCGGCGu -3' miRNA: 3'- cCGCGAaacACaACUGUGGAGUUGUc -5' |
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| 12998 | 3' | -48.8 | NC_003387.1 | + | 13421 | 0.71 | 0.769276 |
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Target: 5'- cGGCGCUcgccgGUG-UGAacUACCUCGGCAa -3' miRNA: 3'- -CCGCGAaa---CACaACU--GUGGAGUUGUc -5' |
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| 12998 | 3' | -48.8 | NC_003387.1 | + | 22810 | 0.73 | 0.680144 |
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Target: 5'- aGUGC----UGUUGAUGCCUCAACAGu -3' miRNA: 3'- cCGCGaaacACAACUGUGGAGUUGUC- -5' |
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| 12998 | 3' | -48.8 | NC_003387.1 | + | 2128 | 0.75 | 0.553407 |
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Target: 5'- cGUGCU----GUUGAUACCUCAACAGu -3' miRNA: 3'- cCGCGAaacaCAACUGUGGAGUUGUC- -5' |
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| 12998 | 3' | -48.8 | NC_003387.1 | + | 23606 | 0.76 | 0.519739 |
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Target: 5'- -cCGCUgggGUGUUGAUACCcCAACAGc -3' miRNA: 3'- ccGCGAaa-CACAACUGUGGaGUUGUC- -5' |
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| 12998 | 3' | -48.8 | NC_003387.1 | + | 47329 | 0.77 | 0.465572 |
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Target: 5'- cGGCGUUUUcGUGUUGACAggUCAACAc -3' miRNA: 3'- -CCGCGAAA-CACAACUGUggAGUUGUc -5' |
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| 12998 | 3' | -48.8 | NC_003387.1 | + | 23635 | 0.79 | 0.376185 |
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Target: 5'- aGGCGCagaacUGUUGGCGCgUCAACAGg -3' miRNA: 3'- -CCGCGaaac-ACAACUGUGgAGUUGUC- -5' |
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| 12998 | 3' | -48.8 | NC_003387.1 | + | 29877 | 0.84 | 0.19876 |
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Target: 5'- gGGCGCUUUcGUGUUGACGggUCAACAGc -3' miRNA: 3'- -CCGCGAAA-CACAACUGUggAGUUGUC- -5' |
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| 12998 | 3' | -48.8 | NC_003387.1 | + | 46388 | 0.86 | 0.140882 |
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Target: 5'- gGGCGCagcGUGUUGACAgCUCAACAGu -3' miRNA: 3'- -CCGCGaaaCACAACUGUgGAGUUGUC- -5' |
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| 12998 | 3' | -48.8 | NC_003387.1 | + | 49970 | 0.93 | 0.052288 |
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Target: 5'- gGGCG-UUUGUGUUGACACCUCAACAc -3' miRNA: 3'- -CCGCgAAACACAACUGUGGAGUUGUc -5' |
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| 12998 | 3' | -48.8 | NC_003387.1 | + | 50559 | 1.13 | 0.002373 |
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Target: 5'- gGGCGCUUUGUGUUGACACCUCAACAGg -3' miRNA: 3'- -CCGCGAAACACAACUGUGGAGUUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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