Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13001 | 3' | -49 | NC_003387.1 | + | 17467 | 0.66 | 0.951503 |
Target: 5'- uGCGCAGcaaGGACgaUCCCGGcgGCAu -3' miRNA: 3'- gCGCGUUaaaUCUG--AGGGCUaaCGUc -5' |
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13001 | 3' | -49 | NC_003387.1 | + | 25854 | 0.77 | 0.414485 |
Target: 5'- aCGCGCGAUgcucAGACUCgCCGAggUGCAu -3' miRNA: 3'- -GCGCGUUAaa--UCUGAG-GGCUa-ACGUc -5' |
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13001 | 3' | -49 | NC_003387.1 | + | 27246 | 0.67 | 0.930611 |
Target: 5'- aCGCGCGGUcgAGGCggCCCGGcaUGCc- -3' miRNA: 3'- -GCGCGUUAaaUCUGa-GGGCUa-ACGuc -5' |
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13001 | 3' | -49 | NC_003387.1 | + | 31122 | 0.68 | 0.909016 |
Target: 5'- aGCGCcAggUGGGCgccgCCCGAgaacaucgagucgUGCAGg -3' miRNA: 3'- gCGCGuUaaAUCUGa---GGGCUa------------ACGUC- -5' |
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13001 | 3' | -49 | NC_003387.1 | + | 39782 | 0.69 | 0.874288 |
Target: 5'- aGCGCAAcg-AGGCcagcggCCUGAUUGCGc -3' miRNA: 3'- gCGCGUUaaaUCUGa-----GGGCUAACGUc -5' |
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13001 | 3' | -49 | NC_003387.1 | + | 51045 | 1.09 | 0.004179 |
Target: 5'- uCGCGCAAUUUAGACUCCCGAUUGCAGc -3' miRNA: 3'- -GCGCGUUAAAUCUGAGGGCUAACGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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