Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13002 | 5' | -59.4 | NC_003387.1 | + | 2496 | 0.67 | 0.442832 |
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Target: 5'- gCGCCUCGuaugugacGGUGacgccGACUGAuGAGGGGCc -3' miRNA: 3'- aGCGGGGC--------UCACc----CUGACU-CUCCUCG- -5' |
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| 13002 | 5' | -59.4 | NC_003387.1 | + | 42769 | 0.67 | 0.433411 |
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Target: 5'- gUGCCCCGAGUcGGGcaagugGCUGGuGuGGAaagGCa -3' miRNA: 3'- aGCGGGGCUCA-CCC------UGACU-CuCCU---CG- -5' |
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| 13002 | 5' | -59.4 | NC_003387.1 | + | 32263 | 0.68 | 0.374304 |
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Target: 5'- cCGUCgCCGAGgccgacaaggcgugGGGGCgc-GAGGAGCa -3' miRNA: 3'- aGCGG-GGCUCa-------------CCCUGacuCUCCUCG- -5' |
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| 13002 | 5' | -59.4 | NC_003387.1 | + | 38047 | 0.69 | 0.330175 |
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Target: 5'- gCGCCCCGAucGGGcggugGCUGAGc-GAGCg -3' miRNA: 3'- aGCGGGGCUcaCCC-----UGACUCucCUCG- -5' |
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| 13002 | 5' | -59.4 | NC_003387.1 | + | 19895 | 0.7 | 0.306627 |
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Target: 5'- cCGCCCCGcGGUGcGGGCUGcgcuGAGauugcuuGAGCu -3' miRNA: 3'- aGCGGGGC-UCAC-CCUGACu---CUC-------CUCG- -5' |
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| 13002 | 5' | -59.4 | NC_003387.1 | + | 52043 | 0.72 | 0.211328 |
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Target: 5'- -gGCCCUGcGUacccugccGGGGCUG-GGGGAGCa -3' miRNA: 3'- agCGGGGCuCA--------CCCUGACuCUCCUCG- -5' |
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| 13002 | 5' | -59.4 | NC_003387.1 | + | 27933 | 0.73 | 0.195527 |
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Target: 5'- gCGCCUCGGGgGGGGCUcGGucGGGGCg -3' miRNA: 3'- aGCGGGGCUCaCCCUGAcUCu-CCUCG- -5' |
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| 13002 | 5' | -59.4 | NC_003387.1 | + | 49653 | 0.74 | 0.158359 |
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Target: 5'- -gGCCCCGAGUGGGuCgacgucGGAGCg -3' miRNA: 3'- agCGGGGCUCACCCuGacucu-CCUCG- -5' |
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| 13002 | 5' | -59.4 | NC_003387.1 | + | 51802 | 1.12 | 0.000234 |
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Target: 5'- aUCGCCCCGAGUGGGACUGAGAGGAGCg -3' miRNA: 3'- -AGCGGGGCUCACCCUGACUCUCCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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