Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13003 | 5' | -55.8 | NC_003387.1 | + | 4496 | 0.7 | 0.418842 |
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Target: 5'- cGGGCGCGacacgaUCGUGCGGGUCac-CUCGg -3' miRNA: 3'- cCUCGUGU------AGCACGUCCGGgauGAGC- -5' |
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| 13003 | 5' | -55.8 | NC_003387.1 | + | 13155 | 0.7 | 0.409472 |
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Target: 5'- cGAGCACcgCGUGCagaAGGCCgaGCgCGa -3' miRNA: 3'- cCUCGUGuaGCACG---UCCGGgaUGaGC- -5' |
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| 13003 | 5' | -55.8 | NC_003387.1 | + | 2414 | 0.71 | 0.373335 |
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Target: 5'- cGGGGCGCAUCGcGUugacGGCCCagccguuCUCGa -3' miRNA: 3'- -CCUCGUGUAGCaCGu---CCGGGau-----GAGC- -5' |
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| 13003 | 5' | -55.8 | NC_003387.1 | + | 34228 | 0.72 | 0.347689 |
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Target: 5'- aGAGCAUG-C-UGCAGGCCCUcggggcGCUCGa -3' miRNA: 3'- cCUCGUGUaGcACGUCCGGGA------UGAGC- -5' |
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| 13003 | 5' | -55.8 | NC_003387.1 | + | 12185 | 0.74 | 0.251435 |
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Target: 5'- gGGGGuCACGcccUCGgGCAGGUCgCUGCUCGg -3' miRNA: 3'- -CCUC-GUGU---AGCaCGUCCGG-GAUGAGC- -5' |
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| 13003 | 5' | -55.8 | NC_003387.1 | + | 42411 | 0.75 | 0.220708 |
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Target: 5'- aGGAGCAguUCGaGCAGGCCCgcaaGgUCGu -3' miRNA: 3'- -CCUCGUguAGCaCGUCCGGGa---UgAGC- -5' |
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| 13003 | 5' | -55.8 | NC_003387.1 | + | 52550 | 0.78 | 0.14719 |
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Target: 5'- cGAGCACcgucuugccGUCGgccaGCAGGCCCaGCUCGg -3' miRNA: 3'- cCUCGUG---------UAGCa---CGUCCGGGaUGAGC- -5' |
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| 13003 | 5' | -55.8 | NC_003387.1 | + | 52069 | 1.11 | 0.000593 |
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Target: 5'- gGGAGCACAUCGUGCAGGCCCUACUCGa -3' miRNA: 3'- -CCUCGUGUAGCACGUCCGGGAUGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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