Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13005 | 5' | -54.4 | NC_003387.1 | + | 48734 | 0.7 | 0.497944 |
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Target: 5'- gCCGCGACGAGuCCAuGACcG-CGCGg-- -3' miRNA: 3'- -GGCGCUGCUC-GGU-CUGaCaGUGCaaa -5' |
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| 13005 | 5' | -54.4 | NC_003387.1 | + | 30182 | 0.7 | 0.487565 |
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Target: 5'- aCCGCGuCGAGCCAGcccucggccuggGCcaggGUCGCGg-- -3' miRNA: 3'- -GGCGCuGCUCGGUC------------UGa---CAGUGCaaa -5' |
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| 13005 | 5' | -54.4 | NC_003387.1 | + | 17176 | 0.71 | 0.464094 |
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Target: 5'- aUCGCGgcgucgugcuccugGCGGGCCAGGUUGUCGCGc-- -3' miRNA: 3'- -GGCGC--------------UGCUCGGUCUGACAGUGCaaa -5' |
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| 13005 | 5' | -54.4 | NC_003387.1 | + | 21479 | 0.71 | 0.437314 |
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Target: 5'- cCCGCGccgguCGGGCCGGGCUGguuCGUg- -3' miRNA: 3'- -GGCGCu----GCUCGGUCUGACaguGCAaa -5' |
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| 13005 | 5' | -54.4 | NC_003387.1 | + | 7462 | 0.72 | 0.399331 |
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Target: 5'- aCGCGAUGGGCCAGGCgaUCACc--- -3' miRNA: 3'- gGCGCUGCUCGGUCUGacAGUGcaaa -5' |
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| 13005 | 5' | -54.4 | NC_003387.1 | + | 52359 | 1.08 | 0.001469 |
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Target: 5'- gCCGCGACGAGCCAGACUGUCACGUUUg -3' miRNA: 3'- -GGCGCUGCUCGGUCUGACAGUGCAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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