Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13008 | 5' | -48.9 | NC_003390.1 | + | 8704 | 0.67 | 0.927971 |
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Target: 5'- aAGUACAaGGUGaUGGAU---CUGACCg -3' miRNA: 3'- -UCGUGUaUCGCaACCUAcauGACUGG- -5' |
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| 13008 | 5' | -48.9 | NC_003390.1 | + | 31833 | 0.67 | 0.915982 |
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Target: 5'- aAGCACGgGGaCGUUGGAUGggugaggugguugcCUGcACCg -3' miRNA: 3'- -UCGUGUaUC-GCAACCUACau------------GAC-UGG- -5' |
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| 13008 | 5' | -48.9 | NC_003390.1 | + | 4093 | 0.67 | 0.91532 |
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Target: 5'- uGGCACccuuGUGGCGcUGGcgGUcagGACCg -3' miRNA: 3'- -UCGUG----UAUCGCaACCuaCAugaCUGG- -5' |
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| 13008 | 5' | -48.9 | NC_003390.1 | + | 3380 | 0.67 | 0.90853 |
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Target: 5'- uGCugGggAGCcUUGGAUGUucCUGGCUg -3' miRNA: 3'- uCGugUa-UCGcAACCUACAu-GACUGG- -5' |
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| 13008 | 5' | -48.9 | NC_003390.1 | + | 41078 | 0.73 | 0.660975 |
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Target: 5'- cGGCugGUAGCGUUGGGg--GCauACCa -3' miRNA: 3'- -UCGugUAUCGCAACCUacaUGacUGG- -5' |
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| 13008 | 5' | -48.9 | NC_003390.1 | + | 934 | 1.14 | 0.001666 |
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Target: 5'- cAGCACAUAGCGUUGGAUGUACUGACCg -3' miRNA: 3'- -UCGUGUAUCGCAACCUACAUGACUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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